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Cell-type specific labelling of glycoproteins in engineered K-562 cells (BOCTAG)

ABSTRACT: We report a strategy termed Bio-Orthogonal Cell line-specific Tagging of Glycoproteins (BOCTAG). Cells are equipped by transfection with an artificial biosynthetic pathway (mut-AGX1/NahK) that transforms an alkyne tagged sugar into the corresponding nucleotide-sugar. Only transfected cells incorporate the bioorthogonal sugar into glycoproteins in the presence of non-transfected cells. The incorporation of alkyne-sugar in glycoproteins allows the installation, by copper(I)-catalyzed reaction, of a biotinylated tag. Glycoproteins are enriched using Streptavidin, and on-bead digestion yielded a peptide fraction that is analysed by mass spectrometry.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Benjamin Schumann

LAB HEAD: Benjamin Schumann

PROVIDER: PXD035430 | Pride | 2022-09-10

REPOSITORIES: pride

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			Action	DRS
	CGB9947A1RW2-40-577256.raw	Raw
	CGB9947A1RW3-40-577283.raw	Raw
	CGB9947A1RW4-40-577284.raw	Raw
	CGB9947A2RW2-40-577257.raw	Raw
	CGB9947A2RW3-40-577285.raw	Raw

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