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Tropodithietic acid, a multifunctional antimicrobial, facilitates adaption and colonization of the producer, Phaeobacter piscinae S26

ABSTRACT: In the marine environment, surface-associated bacteria often produce an array of antimicrobial secondary metabolites (MSMs), which have predominantly been perceived as competition molecules. However, they may also affect other hallmarks of surface-associated living, such as motility and biofilm formation. Here, we investigate the ecological significance of an antibiotic secondary metabolite, tropodithietic acid (TDA), in the producing bacterium, Phaeobacter piscinae S26. We constructed a markerless in-frame deletion mutant deficient in TDA biosynthesis wherein TDA production was abolished. Molecular networking demonstrated that other chemical sulphur-containing features, likely related to TDA, were also altered in the secondary metabolome. We found dramatic changes in the physiology of the TDA-deficient mutant, S26ÃƒÅ½Ã¢â‚¬ï¿½tdaB, compared to the wild type S26. Growth of the two strains were similar; however, S26ÃƒÅ½Ã¢â‚¬ï¿½tdaB cells were shorter and more motile. Transcriptome and proteome profiling revealed an increase in expression of genes and relative abundance of proteins related to a type IV secretion system, a prophage, and a gene transfer agent (GTA) in S26ÃƒÅ½Ã¢â‚¬ï¿½tdaB. All these systems may contribute to horizontal gene transfer (HGT), which may facilitate fast adaptation to novel niches. We speculate that, once a TDA-producing population has been established in a new niche, the accumulation of TDA acts as a signal of successful colonization, prompting a switch to a sessile lifestyle. This would lead to a decrease in motility and the rate of HGT, whilst filamentous cells could form the base of a biofilm. In addition, the antibiotic properties of TDA may inhibit invading competing microorganisms.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Phaeobacter Piscinae

TISSUE(S): Secretion, Cell Culture

SUBMITTER: Shengda Zhang

LAB HEAD: Lone Gram

PROVIDER: PXD035448 | Pride | 2023-01-01

REPOSITORIES: Pride

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			Action	DRS
	20210114_KMJ_MWN_LauraLL_140min_260ng_3.raw	Raw
	20210114_KMJ_MWN_LauraLL_140min_500ng_1.raw	Raw
	20210114_KMJ_MWN_LauraLL_140min_500ng_10.raw	Raw
	20210114_KMJ_MWN_LauraLL_140min_500ng_11.raw	Raw
	20210114_KMJ_MWN_LauraLL_140min_500ng_12.raw	Raw

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Tropodithietic acid, a multifunctional antimicrobial, facilitates adaption and colonization of the producer, Phaeobacter piscinae S26

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