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Molecular study of the Phyto-Courier Nanotechnology in Salt-Stressed Tomato

ABSTRACT: The search for more effective methods to alleviate the negative effects of abiotic stresses in plants has motivated the experimentation of new technologies, namely nanotechnologies. It is in this context that the use of formulations containing hybrid silicon (Si) nanoparticles (NPs) and acting as delivery systems of the flavonoid quercetin was here investigated. These formulations, previously referred to as phyto-couriers, proved their efficacy in protecting textile hemp against salinity. We here broaden their application spectrum by studying the effects on an important crop model, tomato (Solanum lycopersicum cv. Micro-Tom). Two phyto-courier formulations, labelled GS1 and GS3, functionalized with 25 mg of quercetin and differing in the presence of trehalose were applied to salt-stressed plants (200 mM NaCl) by foliar spraying. Microscopy showed, under stress, a preservation of the palisade mesophyll cell structure in plants treated with the formulations. A trend towards decreased expression of some stress-responsive genes was observed in the leaves treated with the phyto-couriers. Shotgun proteomics confirmed the protective effects and the nano-biostimulant nature of the formulations, since several proteins involved in cytoprotection against oxidative stress were more abundant in control leaves treated with the pyto-courier. This was especially evident for the trehalose-containing GS3 formulation. Some proteins involved in chromatin remodelling were also more abundant in control leaves treated with GS3. Overall, the formulations showed promising results to enhance abiotic stress tolerance in a model crop through the mitigation of stress symptoms. The results presented are a proof-of-concept for the use of the phyto-courier nanotechnology in horticultural applications

INSTRUMENT(S): TripleTOF 6600

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Céline LECLERCQ

LAB HEAD: Céline LECLERCQ

PROVIDER: PXD035460 | Pride | 2023-03-06

REPOSITORIES: Pride

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			Action	DRS
	20210421_Sapphyre_align_exportMSMS.mgf	Mgf
	20220718_Sapphyre.MSMS.mgf	Mgf
	20220718_Sapphyre.mzid.gz	Mzid
	20220718_Sapphyre_resume_samples.xlsx	Xlsx
	F174409.dat	Other

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Project description:Yield losses as a result of abiotic stress factors present a significant challenge for the future of global food production. While breeding technologies provide potential to combat negative stress-mediated outcomes over time, interventions which act to prime plant tolerance to stress, via the use of phytohormone-based elicitors for example, could act as a valuable tool for crop protection. However, the translation of fundamental biology into functioning solution is often constrained by knowledge-gaps. Photosynthetic and transcriptomic responses were characterised in young tomato (Solanum lycopersicum) seedlings in response to pre-treatment with a new plant health activator technology, M-bM-^@M-^XAlethea,M-bM-^@M-^Y followed by a subsequent 100 mM salinity stress. Salinity treatment led to a maximal 47% reduction in net photosynthetic rate 8 d following NaCl treatment. In Alethea pre-treated seedlings, sensitivity to salinity stress was markedly reduced during the experimental period. Microarray analysis of leaf transcriptional responses showed that while salinity stress and Alethea individually impacted on largely non-overlapping, distinct groups of genes, Alethea pre-treatment substantially modified the response to salinity. Alethea affected the expression of genes related to biotic stress, ethylene signalling, cell wall synthesis, redox signalling and photosynthetic processes. Since Alethea had clear effects on photosynthesis/chloroplastic function at the physiological and molecular levels, we also investigated the ability of Alethea to protect plants against methyl viologen, a potent generator of oxidative stress in chloroplasts. Alethea pre-treatment produced dramatic reductions in visible foliar necrosis caused by methyl viologen compared with non-primed controls. Tomato leaves from four groups of treated plants were harvested at a single point following treatment for RNA extraction and hybridization on Affymetrix microarrays. Plants came from two initial groups, pre-treated either with water (control) or the Alethea product. Equal numbers of plants from within these groups were subsequently treated either with water (control) or salinity (100 mM NaCl), generating 4 main experimental groups in a 2x2 factorial design. Samples were collected from 3 biological replicate experiments, and each sample included leaves pooled from 3 individual plants from each treatment group.

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Molecular study of the Phyto-Courier Nanotechnology in Salt-Stressed Tomato

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