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Immunization of mice with virus-like vesicles of Kaposi sarcoma-associated herpesvirus reveals a role of ORF4 in complement-mediated neutralization

ABSTRACT: The development of a prophylactic vaccine for Kaposi sarcoma-associated Herpesvirus (KSHV) would prevent consequences from infection including disorders such as Kaposi sarcoma and primary effusion lymphoma. Here, we study the immunogenicity of noninfectious virus-like vesicles (VLVs) of KSHV as a potential future vaccine platform. VLVs present a repertoire of viral structural proteins but are noninfectious due to a defect in capsid formation that prevents viral DNA packaging. Immunization of mice with adjuvanted VLVs results in virus-specific antibodies and T cells. These antibodies neutralize viral infection, and this neutralization is enhanced by the complement system. Complement-enhanced neutralization is dependent on antibodies targeting the SCR region of viral ORF4. However, this activity was not present in serum from KSHV-infected humans. Our study highlights an important role of antibody effector functions in the development of a future KSHV vaccine

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human) Human Gammaherpesvirus 8

TISSUE(S): Epithelial Cell

SUBMITTER: Jeffrey Johnson

LAB HEAD: Jeffrey Johnson

PROVIDER: PXD035478 | Pride | 2023-02-01

REPOSITORIES: pride

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			Action	DRS
	20191010.SwissProt.Hsapiens.fasta	Fasta
	20220407_113047_20220405-TT01_Report.tsv	Tabular
	EC20220330-13.raw	Raw
	EC20220330-14.raw	Raw
	EC20220330-15.raw	Raw

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