Dataset Information

Early Secretory Pathway glycoprotein analysis dataset

ABSTRACT: Protein misfolding is one of the causes for several diseases and as a consequence, analysis of the misfolded protein fraction is one of the major aims in translational research. Here, we report a workflow and its associated dataset which focuses on the analysis of the Early Secretory Pathway (ESP) glycoproteins (gESP) from melanoma cells. Our results provide an overview on the ESP glycoprotein members by analysis of both the peptide and glycan chains. Moreover we provide the quantitative analysis of cells treated with kifunensine (an immunomodulatory agent), which blocks class I mannosidases processing of the misfolded or unfolded glycoproteins sent to degradation. Our dataset provides an overview of the major cargo of ESP glycoproteins, underpinning novel candidates for glycoprotein-related Endoplasmic Reticulum Associated Degradation (ERAD) which could be involved in melanoma antigen presentation.

INSTRUMENT(S): LTQ Orbitrap Velos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Melanocyte

DISEASE(S): Melanoma

SUBMITTER: Cristian Munteanu

LAB HEAD: Ștefana M. Petrescu

PROVIDER: PXD035541 | Pride | 2023-01-31

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	180616_04ST-CTRGluC_GlycoPepsHCD_1290ng_oncolumn_run01.raw	Raw
	180617_04ST-CTRGluC_GlycoPepsHCDExList_1290ng_oncolumn_run03.raw	Raw
	180617_04ST-CTRGluC_GlycoPepsHCDExList_1290ng_oncolumn_run05.raw	Raw
	180618_04ST-KIFGluC_GlycoPepsHCD_1591ng_oncolumn_run01.raw	Raw
	180619_04ST-KIFGluC_GlycoPepsHCDExList_2150ng_oncolumn_run03.raw	Raw

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Publications

Defining the altered glycoproteomic space of the early secretory pathway by class I mannosidase pharmacological inhibition.

Munteanu Cristian V A CVA Chirițoiu Gabriela N GN Petrescu Andrei-Jose AJ Petrescu Ștefana M ȘM

Frontiers in molecular biosciences 20230109

N-glycosylation is a key process for various biological functions like protein folding, maturation and sorting for the conventional secretory compartment, cell-cell communication and immune response. This is usually accomplished by a complex system of mannosidases in which those from class I have an outstanding role, commonly involved in the early protein sorting associated to the Endoplasmic Reticulum (ER) in the N-glycan dependent quality control (ERQC) and ER-associated degradation (ERAD). Al ...[more]

PMID: 36699698

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