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Proteomic study on the lymphocytes from pregnant Wistar rats females treated with immunosuppressive regimen

ABSTRACT: Kidney transplantation remains the optimal therapeutical option for end stage kidney disease. Current immunosuppressive regimens are efficient in combating acute kidney rejection. However, the insight in chronic kidney allograft injury remains limited. Simultaneously, pregnancy in kidney recipients appears more prevalent than during dialysis treatment. Due to ethical issues comprehensive studies on the impact of current immunosuppressive regimens on pregnancy are challenging. Omics- technology enables the systemic insight into biology both of immunity and pregnancy beyond up-to-date collected knowledge. The aim of the study was to investigate the proteomic status of lymphocytes obtained from pregnant female rats under immunosuppressive treatment. The group of 10 pregnant Wistar rat females, 5 of which treated with tacrolimus, mofetil mycophenolate and glicocorticosteroids, 5 used as control. The lymphocytes were obtained and analyzed with mass-spectrometry. The outcomes were verified statistically in terms of proteins up- and down regulation.

INSTRUMENT(S): TripleTOF 5600+

ORGANISM(S): Rattus Norvegicus (rat)

TISSUE(S): Lymphocyte

SUBMITTER: Aleksandra Bogucka

LAB HEAD: Paulina Czaplewska

PROVIDER: PXD035571 | Pride | 2023-03-11

REPOSITORIES: Pride

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Proteomic study on the lymphocytes from pregnant Wistar rat females treated with immunosuppressive regimen.

Wojciuk Bartosz B Bogucka Aleksandra A Czaplewska Paulina P Okulewicz Patrycja P Wojciechowska-Koszko Iwona I Ciechanowski Kazimierz K Kabat-Koperska Joanna J

Clinical and translational science 20221110 1

Kidney transplantation remains the therapeutic option for patients with end-stage kidney disease. Current immunosuppressive regimens are efficient in combating acute kidney rejection. However, insights into chronic kidney allograft injury remains limited. Simultaneously, pregnancy is more common after kidney transplantation than during dialysis treatment. Due to ethical issues, comprehensive studies on the impact of immunosuppressive regimens on pregnancy are challenging. The study aimed to inve ...[more]

PMID: 36366854

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