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Structural surfaceomics of Nomo-1 using DSSO cross-linker with MS3 approach

ABSTRACT: The project aimed to profile the cell surface proteins of Nomo-1 (AML cell line) using structural surfaceomics for identification of protein conformation-based cancer antigens thereby expanding the toolkit for cancer target discovery for immunotherapeutic targeting. To achieve the goal, cell surface capture (CSC) was integrated with cross-linking mass spectrometry (XL-MS). DSSO was used as a cross-linker to freeze the structural conformations of protein in three-dimensional space, followed by biotinylation of cell surface proteins to enable enrichment of cell surface proteins to allow focused XL-MS analysis of those enriched proteins. DSSO being MS cleavable cross-linker, allowed higher order MS3 approach for analysis.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Myeloid Cell Line, Myeloid Cell

DISEASE(S): Acute Myeloid Leukemia

SUBMITTER: Kamal Mandal

LAB HEAD: Dr. Arun P. Wiita

PROVIDER: PXD035589 | Pride | 2023-10-04

REPOSITORIES: Pride

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