Wetland four-member synthetic community
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ABSTRACT: The synthetic microbial community used in this study was composed of the major functional guilds (cellulolytic fermenter, sulfate reducer, hydrogenotrophic methanogen and acetoclastic methanogen) that mediate the anaerobic conversion of cellulosic biomass to CH4 and CO2 in wetland soils. The choice of a facultative sulfate-reducing bacterium (Desulfovibrio vulgaris Hildenborough) introduced metabolic versatility and enabled investigations into the community response to sulfate intrusion. The growth status of these multi-species cultures was measured over a week by daily analysis of substrate consumption and product accumulation. The quad-cultures were analyzed with metaproteomics at the end of experiment to characterize the community structure and metabolic activities.
INSTRUMENT(S): LTQ Orbitrap Elite
ORGANISM(S): Desulfovibrio Vulgaris Str. Hildenborough Methanospirillum Hungatei Jf-1 Clostridium Cellulolyticum (strain Atcc 35319 / Dsm 5812 / Jcm 6584 / H10) Methanothrix Soehngenii Gp6
TISSUE(S): Cell Culture
SUBMITTER: Dongyu Wang
LAB HEAD: Chongle Pan
PROVIDER: PXD035759 | Pride | 2023-02-17
REPOSITORIES: Pride
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