Proteomics

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Wetland four-member synthetic community


ABSTRACT: The synthetic microbial community used in this study was composed of the major functional guilds (cellulolytic fermenter, sulfate reducer, hydrogenotrophic methanogen and acetoclastic methanogen) that mediate the anaerobic conversion of cellulosic biomass to CH4 and CO2 in wetland soils. The choice of a facultative sulfate-reducing bacterium (Desulfovibrio vulgaris Hildenborough) introduced metabolic versatility and enabled investigations into the community response to sulfate intrusion. The growth status of these multi-species cultures was measured over a week by daily analysis of substrate consumption and product accumulation. The quad-cultures were analyzed with metaproteomics at the end of experiment to characterize the community structure and metabolic activities.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Desulfovibrio Vulgaris Str. Hildenborough Methanospirillum Hungatei Jf-1 Clostridium Cellulolyticum (strain Atcc 35319 / Dsm 5812 / Jcm 6584 / H10) Methanothrix Soehngenii Gp6

TISSUE(S): Cell Culture

SUBMITTER: Dongyu Wang  

LAB HEAD: Chongle Pan

PROVIDER: PXD035759 | Pride | 2023-02-17

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Pan_102621_Q1_SF05.mzML Mzml
Pan_102621_Q1_SF05.raw Raw
Pan_102621_Q1_SF06.mzML Mzml
Pan_102621_Q1_SF06.raw Raw
Pan_102621_Q1_SF07.mzML Mzml
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