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Multi-omics analysis of application on regulation of phosphorus transport in soybean root

ABSTRACT: The regulatory effects of uneven phosphorus supplies on phosphorus transport in soybean roots is still unclear. To further analyze the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots and the effects of uneven phosphorus application on the physiological mechanism of phosphorus transport in soybean roots, dual-root soybean plants were prepared via grafting, and a sand culture experiment was performed.

INSTRUMENT(S): 6410 Triple Quadrupole LC/MS

ORGANISM(S): Acyrthosiphon Pisum (pea Aphid)

TISSUE(S): Root

SUBMITTER: Hongyu LI

LAB HEAD: Li Hongyu

PROVIDER: PXD035894 | Pride | 2022-10-14

REPOSITORIES: Pride

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			Action	DRS
	P20210803309.msf	Msf
	P20210803309_F1.raw	Raw
	P20210803309_F10.raw	Raw
	P20210803309_F2.raw	Raw
	P20210803309_F3.raw	Raw

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Multi-omics analysis of the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots.

Li Hongyu H Xu Letian L Li Jiaxin J Lyu Xiaochen X Li Sha S Wang Chang C Wang Xuelai X Ma Chunmei C Yan Chao C

Frontiers in plant science 20220902

The regulatory effects of uneven phosphorus supplies on phosphorus transport in soybean roots are still unclear. To further analyze the regulatory effects of low-phosphorus stress on phosphorus transport in soybean roots and the effects of uneven phosphorus application on the physiological mechanism of phosphorus transport in soybean roots, dual-root soybean plants were prepared via grafting, and a sand culture experiment was performed. From the unfolded cotyledon stage to the initial flowering ...[more]

PMID: 36119614

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Biophoton emission is involved in reactive oxygen species as well as mitochondrial proteins

Project description:Mitochondrial Proteomics under flooding stress in roots of soybean plants

2016-08-10 | PXD001477 | Pride

Identification of differential phosphoproteins reveled complex responses of soybean to phosphate starvation through reversible protein phosphorylation

Project description:Phosphorus (P) deficiency is considered as a major constraint on crop production. Although a set of adaptative strategies are extensively suggested in soybean (Glycine max) to phosphate(Pi) deprivation, molecular mechanisms underlying reversible protein phosphorylation in soybean responses to P deficiency remains largely unclear. In this study, isobaric tags for relative and absolute quantitation, combined with liquid chromatography and tandem mass spectrometry analysis was performed to identify differential phosphoproteins in soybean roots under Pi sufficient and deficient conditions. A total of 427 phosphoproteins were found to exhibit differential accumulations, with 213 up-regulated and 214 down-regulated.Taken together, identification of differential phosphoproteins not only reveled complex regulatory pathways in soybean adaptation to Pi starvation through reversible protein phosphorylation.

2021-06-13 | PXD025771 | Pride

Genome wide transcriptome analysis reveals complex regulatory mechanisms underlying phosphate homeostasis in soybean nodules

Project description:Phosphorus (P) deficiency is a major limitation for legume crop production. Although overall adaptations of plant roots to P deficiency have been extensively studied, fragmentary information is available in regards to root nodule responses to P deficiency. In this study, genome wide transcriptome analysis was conducted using RNA-seq analysis to investigate molecular mechanisms underlying soybean (Glycine max) nodule adaptation to phosphate (Pi) starvation. Phosphorus deficiency significantly decreased soybean nodule growth and nitrogenase activity. Nodule Pi concentrations declined by 49% in response to P deficiency, but this was well below the 87% and 88% decreases observed in shoots and roots, respectively. Nodule transcript profiling revealed that a total of 2,055 genes exhibited differential expression patterns between Pi sufficient and deficient conditions. A set of DEGs appeared to be involved in maintaining Pi homeostasis in soybean nodules, including 8 Pi transporters (PTs), 8 proteins containing the SYG1/PHO81/XPR1 domain (SPXs), and 16 purple acid phosphatases (PAPs). The results suggest that a complex transcriptional regulatory network participates in soybean nodule adaption to Pi starvation, most notable a Pi signaling pathway specifically involved in maintaining Pi homeostasis in nodules.

2018-11-20 | GSE116593 | GEO

Expression data of soybean accessions under different phosphorus treatments

Project description:Phosphorus is one of the most important macronutrients that is required for plant growth and development. However, stress under low-P conditions has become a limiting factor that affects crop yields and qualities. Plants have developed strategies to cope with this, while few genes associated with low-P tolerance have been identified in soybean. We used microarrays to detail the global programme of gene expression under different phosphorus treatments of two soybean accessions CD and YH with different phosphorus efficiency. The roots and leaves of a low-P-tolerant accession and a low-P-sensitive accession were harvested after 10 days of hydroponics under different P treatments, each with three biological replicates.Then microarray chips were performed on the 24 samples. We sought to identify genes associated with low-P stress. To that end, we analyzed the differently expressed genes between different P treatments, different accessions and different tissues.

2016-03-08 | E-GEOD-78242 | biostudies-arrayexpress

Proteome analysis of the soybean nodule phosphorus response mechanism and characterisation of stress-induced ribosome structural and protein expression changes

Project description:In agroecosystems, a plant-usable form of nitrogen is mainly generated by legume-based biological nitrogen fixation, a process that requires phosphorus (P) as an essential nutrient. To investigate the physiological mechanism whereby phosphorus influences soybean nodule nitrogen fixation, soybean root nodules were exposed to four phosphate levels: 1 mg/L (P stress), 11 mg/L (P stress), 31 mg/L (Normal P), 61 mg/L (High P) then proteome analysis of nodules was conducted to identify phosphorus-associated proteome changes. We found that phosphorus stress-induced ribosomal protein structural changes were associated with altered key root nodule protein synthesis profiles. Importantly, up-regulated expression of peroxidase was observed as an important phosphorus stress-induced nitrogen fixation-associated adaptation that supported two nodule-associated activities: scavenging of reactive oxygen species (ROS) and cell wall growth. In addition, phosphorus transporter (PT) and purple acid phosphatase (PAPs) were up-regulated that regulated phosphorus transport and utilisation to maintain phosphorus balance and nitrogen fixation function in phosphorus-stressed root nodules.

2022-08-11 | PXD033875 | Pride

Complex regulatory mechanisms underlying mineral nutrient acquisition in soybean roots responsive to low pH stress

Project description:Proton toxicity is one of the major environmental stresses limiting crop production, and becomes increasingly serious because of anthropogenic activities. To understand acid tolerance mechanisms, the plant growth, mineral nutrient accumulation and global transcriptome changes in soybean (Glycine max) in response to long-term acid stress were investigated. Results showed that acid stress significantly inhibited soybean root growth, but exhibited slight effects on the shoot growth. Moreover, concentrations of essential mineral nutrients were significantly affected by acid stress, mainly dependent on soybean organs and mineral nutrient types. The concentrations of phosphorus (P) and molybdenum (Mo) in both leaves and roots, nitrogen (N) and potassium (K) in roots and magnesium (Mg) in leaves were significantly decreased, respectively. Whereas, the concentrations of calcium (Ca), sulfate (S) and iron (Fe) were increased in both leaves and roots. Transcriptome analyses in soybean roots resulted in identifying 419 up-regulated and 555 down-regulated genes under acid conditions. A total of 38 differentially expressed genes (DEGs) were involved in mineral nutrient transportation. Among them, all the detected five GmPTs and GmZIPs, two GmAMTs and GmKUP genes, together with GmIRT1, GmNramp5, GmVIT2.1, GmSKOR, GmTPK5 and GmHKT1, were significantly suppressed. Moreover, the genes encoding transcription factors (e.g., GmSTOP2s and a GmPHL1), and genes involved in pH stat metabolic pathways were significantly up-regulated by low pH stress in soybean roots. Taken together, it strongly suggested that maintaining pH stat and mineral nutrient homeostasis are adaptive strategies of soybean responses to acid stress, which might be regulated by a complex signaling network.

2019-06-24 | GSE129320 | GEO

Gene expression data of winter wheat root under drought stress for 7 days by two phosphorus treatments

Project description:A complex regulatory network is the mechanism of wheat roots responding to drought stress at low and adequate phosphorus levels. The transcription levels of genes encoding silicon transporters, phosphate transporters, sucrose synthesis, etc., are mostly up-regulated in Xindong20. The genes encoding the electron transport chain and the respiratory chain are mostly down-regulated in Xindong23. These results suggest that wheat roots should maintain the structural integrity of the cells and reduce the energy metabolism during the coupled stress of drought and low phosphorus, which will help to improve the drought tolerance of wheat. The objective of the present data was to increase the information about the effect of drought on the transcriptomes of wheat root cultured by two phosphorus levels.

2021-11-29 | GSE189698 | GEO

Insights into the proteomic response of soybean to stress of Al2O3, ZnO, and Ag nanoparticles

Project description:The present study highlights the phytotoxicity of nanoparticles (NPs) to soybean roots and leaves at proteome level. The effect of three NPs Al2O3, ZnO, and Ag suspensions on soybean was evaluated.

2015-11-16 | PXD001923 | Pride

Comparative microarray analysis of NaV1.8-expressing DRG neurons and cold-sensitive non-NaV1.8-expressing DRG neurons

Project description:The vast majority of cold sensitive DRG neurons from mice do not express the voltage-gated sodium channel NaV1.8. Therefore, we aimed to compare the molecular profiles of NaV1.8 and non-NaV1.8-expressing neurons using microarray analysis. Fluorescent activated cell sorting was performed at 4 degrees centigrade on acutely dissociated DRG neurons from mice expressing NaV1.8-Cre, Pirt-GCaMP3 and a Cre-dependent global reporter (td tomato). NaV1.8-expressing neurons were sorted based on their reporter fluorescence (td tomato; red) and putative cold sensing neurons were sorted based on their GCaMP3 fluorescence at 4 degrees centigrade and absence of Cre-dependent reporter fluorescence. A total of three mice were used (samples one, two and three) with GCaMP3 only and NaV1.8-expressing neurons forming two relative populations within each sample (eg. GC3 one is the experimental counterpart of Tom one).

2019-07-01 | E-MTAB-7070 | biostudies-arrayexpress

Soybean, root-rhizobia, LC-MSMS

Project description:We previously found that rhizobia-inoculation enhanced the soybean's salt tolerance; the transcription factor (TF) GmMYB173 and its downstream gene GmCHS5 dictate soybean’s flavonoid metabolism in response to this stress. For revealing the mechanism of the enhancement, quantitative phosphoproteomics and metabonomic approaches were used to identify phosphoproteins and metabolites that dominant the common pathway between salinity and rhizobia induced response in soybean roots.

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