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Proteomic profiling of subsets of human primary NK cell-derived extracellular vesicles by LC-MS/MS

ABSTRACT: In this study we have compared the proteomic profile of subsets of extracellular vesicles (EVs) prepared from primary human NK cells cultured for 48hrs in serum-free conditions supplemented with 10 ng/ml human recombinant IL-12, IL-15, and IL-18. The aim was to isolate and define an EV subset with cytolytic activity against tumor cells. For this purpose, bulk EVs were separated according to density (density gradient ultracentrifugation; DG-UC) to yield 3 distinct subsets.

INSTRUMENT(S): timsTOF fleX

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Tuula Nyman

LAB HEAD: Tuula Nyman

PROVIDER: PXD035977 | Pride | 2022-09-09

REPOSITORIES: Pride

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	LC-MS_files.zip	Other
	checksum.txt	Txt
	mqpar.xml	Xml
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