Dataset Information

Proteomic evaluation of human osteoblast responses to titanium implants over time

ABSTRACT: Titanium is widely used in bone prostheses due to its excellent biocompatibility and osseointegration capacity. To understand the effect of sandblasted acid-etched (SAE) Ti implants on the biological responses of human osteoblast (HOb), their proteomic profiles were analysed using nLC-MS/MS. The cells were cultured with the implant materials, and 2544 distinct proteins were detected in samples taken after 1, 3 and 7 days. Comparative analyses of proteomic data were performed using Perseus software. The expression of proteins related to EIF2, mTOR, insulin-secretion and IGF pathways showed marked differences in cells grown with SAE-Ti in comparison with cells cultured without Ti. Moreover, the proteomic profiles obtained with SAE-Ti were compared over time. The affected proteins were related to adhesion, immunity, oxidative stress, coagulation, angiogenesis, osteogenesis and extracellular matrix formation functions. The proliferation, mineralisation and osteogenic gene expression in HObs cultured with SAE-Ti were characterised in vitro. The results showed that the osteoblasts exposed to this material increase their mineralisation rate and expression of COLI, RUNX2, SP7, CTNNB1, CAD13, IGF2, MAPK2 and mTOR. Overall, the observed proteomic profiles can explain the SAE-Ti osteogenic properties, widening our knowledge of key signalling pathways taking part in the early stages of the osseointegration process in this type of implantations.

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Osteoblast

SUBMITTER: Mikel Azkargorta

LAB HEAD: Felix Elortza

PROVIDER: PXD035986 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	210611_210421D_JJSuay_Cells_Cleanup_100_S1-F4_1_2980.d.7z	Other
	210611_210421D_JJSuay_Cells_Cleanup_101_S1-F5_1_2981.d.7z	Other
	210611_210421D_JJSuay_Cells_Cleanup_102_S1-F6_1_2982.d.7z	Other
	210611_210421D_JJSuay_Cells_Cleanup_103_S1-F7_1_2983.d.7z	Other
	210611_210421D_JJSuay_Cells_Cleanup_104_S1-F8_1_2984.d.7z	Other

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Proteomic evaluation of human osteoblast responses to titanium implants over time.

Romero-Gavilán Francisco F Cerqueira Andreia A García-Arnáez Iñaki I Azkargorta Mikel M Elortza Félix F Gurruchaga Mariló M Goñi Isabel I Suay Julio J

Journal of biomedical materials research. Part A 20220830 1

Titanium is widely used in bone prostheses due to its excellent biocompatibility and osseointegration capacity. To understand the effect of sandblasted acid-etched (SAE) Ti implants on the biological responses of human osteoblast (HOb), their proteomic profiles were analyzed using nLC-MS/MS. The cells were cultured with the implant materials, and 2544 distinct proteins were detected in samples taken after 1, 3, and 7 days. Comparative analyses of proteomic data were performed using Perseus softw ...[more]

PMID: 36054528

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