Dataset Information

Profiling the heme-binding proteomes of bacteria using chemical proteomics

ABSTRACT: Heme is a cofactor with myriad roles and is essential to almost all living organisms. Accordingly, bacte-rial pathogens have developed numerous mechanisms to acquire heme from their hosts. Beyond classi-cal gas transport and catalytic functions, heme is increasingly appreciated as a tightly controlled signal-ling molecule regulating protein expression. However, heme acquisition, biosynthesis and regulation is poorly understood beyond a few model organisms, and the heme-binding proteome has yet to be fully characterised in bacteria. Yet as heme homeostasis is critical for bacterial survival, heme-binding pro-teins are promising drug targets. Herein we report a chemical proteomics method for global profiling of heme-binding proteins in live cells for the first time. Employing a panel of heme-based clickable and photoaffinity probes enabled the profiling of 32-54% of the known heme-binding proteomes in Gram-positive and Gram-negative bacteria, and revealed previously unknown potential heme interactors. This simple-to-implement profiling strategy could be interchangeably applied to different cell types and sys-tems and fuel future research into heme biology.

INSTRUMENT(S): Orbitrap Fusion, Q Exactive HF

ORGANISM(S): Enterococcus Faecalis (streptococcus Faecalis) Escherichia Coli

SUBMITTER: Isabel Wilkinson

LAB HEAD: Stephan A. Sieber

PROVIDER: PXD036133 | Pride | 2023-12-05

REPOSITORIES: Pride

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			Action	DRS
	20210205_210119_IW_G_D1.raw	Raw
	20210205_210119_IW_G_D2.raw	Raw
	20210205_210119_IW_G_D3.raw	Raw
	20210205_210119_IW_G_HC_1.raw	Raw
	20210205_210119_IW_G_HC_2.raw	Raw

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Publications

Profiling the Heme-Binding Proteomes of Bacteria Using Chemical Proteomics.

Wilkinson Isabel V L IVL Bottlinger Max M El Harraoui Yassmine Y Sieber Stephan A SA

Angewandte Chemie (International ed. in English) 20230123 9

Heme is a cofactor with myriad roles and essential to almost all living organisms. Beyond classical gas transport and catalytic functions, heme is increasingly appreciated as a tightly controlled signalling molecule regulating protein expression. However, heme acquisition, biosynthesis and regulation is poorly understood beyond a few model organisms, and the heme-binding proteome has not been fully characterised in bacteria. Yet as heme homeostasis is critical for bacterial survival, heme-bindin ...[more]

PMID: 36495310

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