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Protein co-purifying with MLF2-MBP from Expi293 cells

ABSTRACT: MLF2-MBP was expressed as a fusion protein in Expi293 cells. This was purified and a protein around 70 kDa was found to stoichiometrically co-purify. To verify this protein's identity, the 70 kDa region was extracted from a gel in addition to submitting the entire elution for mass spectrometry analysis.

INSTRUMENT(S): LTQ Orbitrap Elite

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Sarah Prophet

LAB HEAD: Christian Schlieker

PROVIDER: PXD036267 | Pride | 2022-08-25

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	MLF2-MBP_Expi_purification.mzid.gz	Mzid
	MLF2-MBP_Expi_purification.mzid_QEp22-0412_Prophet_MLF2-MBP_HSP70_band.MGF	Mzid
	MLF2-MBP_Expi_purification.mzid_QEp22-0417_Prophet_Whole_MLF2-MBP_FLAG_elution.MGF	Mzid
	QEp22-0412_Prophet_MLF2-MBP_HSP70_band.raw	Raw
	QEp22-0417_Prophet_Whole_MLF2-MBP_FLAG_elution.raw	Raw

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