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Mass spectrometry analysis of the neutrophil secretome (Gelatinase/Specific granules)

ABSTRACT: Mass spectrometry analysis of the secretome of wild-type and Wash-cKO Washc1haemo) neutrophils treated with fMLF that favors Gelatinase/Specific granule secretion over azurophilic granule secretion.

INSTRUMENT(S): LTQ Orbitrap

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Neutrophil, Blood

DISEASE(S): Inflammation

SUBMITTER: Sergio Catz

LAB HEAD: Sergio Daniel Catz

PROVIDER: PXD036270 | Pride | 2022-10-13

REPOSITORIES: pride

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Dataset's files

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			Action	DRS
	3493_WT_MZID.mzid	Mzid
	3493_WT_data.dat	Other
	3494_Wash-cKO_MZID.mzid	Mzid
	3494_Wash-cKO_data.dat	Other

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Publications

Differential dysregulation of granule subsets in WASH-deficient neutrophil leukocytes resulting in inflammation.

Johnson Jennifer L JL Meneses-Salas Elsa E Ramadass Mahalakshmi M Monfregola Jlenia J Rahman Farhana F Carvalho Gontijo Raquel R Kiosses William B WB Pestonjamasp Kersi K Allen Dale D Zhang Jinzhong J Osborne Douglas G DG Zhu Yanfang Peipei YP Wineinger Nathan N Askari Kasra K Chen Danni D Yu Juan J Henderson Scott C SC Hedrick Catherine C CC Ursini Matilde Valeria MV Grinstein Sergio S Billadeau Daniel D DD Catz Sergio D SD

Nature communications 20220921 1

Dysregulated secretion in neutrophil leukocytes associates with human inflammatory disease. The exocytosis response to triggering stimuli is sequential; gelatinase granules modulate the initiation of the innate immune response, followed by the release of pro-inflammatory azurophilic granules, requiring stronger stimulation. Exocytosis requires actin depolymerization which is actively counteracted under non-stimulatory conditions. Here we show that the actin nucleator, WASH, is necessary to maint ...[more]

PMID: 36130971

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