Proteomics

Dataset Information

0

Arterial-Venous Endothelial Cell Cycle Proteins Differential Expression

ABSTRACT: HUVEC-FUCCI cells were used to demonstrate that different endothelial cell cycle states provide distict windows of opportunity for gene expression in response to extrinsic signals. HUVEC-FUCCI were FACS-isolated into three different cell cycle states. Peptide digests from the resulting lysates showed differentially expressed proteins among the three cell cycles. These studies show that endothelial cell cycle state determines the propensity for arterial vs. venous fate specification.

INSTRUMENT(S): Orbitrap Eclipse

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Endothelial Cell Of Umbilical Vein, Cell Culture

DISEASE(S): Cardiovascular System Disease

SUBMITTER: Erin Jeffery  

LAB HEAD: Dr. Gloria Sheynkman

PROVIDER: PXD036326 | Pride | 2022-10-12

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
210129_HirschiAllEC_5uL_HCDETD_noFAIMS_Run1.mzXML Mzxml
210129_HirschiAllEC_5uL_HCDETD_noFAIMS_Run1.raw Raw
210129_HirschiAllEC_5uL_HCDETD_noFAIMS_Run2.mzXML Mzxml
210129_HirschiAllEC_5uL_HCDETD_noFAIMS_Run2.raw Raw
210129_HirschiAllEC_5uL_HCDETD_noFAIMS_Run3.mzXML Mzxml
Items per page:
1 - 5 of 29
altmetric image

Publications

During blood vessel development, endothelial cells become specified toward arterial or venous fates to generate a circulatory network that provides nutrients and oxygen to, and removes metabolic waste from, all tissues. Arterial-venous specification occurs in conjunction with suppression of endothelial cell cycle progression; however, the mechanistic role of cell cycle state is unknown. Herein, using Cdh5-CreER<sup>T2</sup>;R26FUCCI2aR reporter mice, we find that venous endothelial cells are enr  ...[more]

PMID: 36202789

Publication: 1/2

Similar Datasets

Proteomics Exosomes define a local and systemic communication network in healthy pancreas and in pancreatic ductal adenocarcinoma
2023-12-12 | PXD047009 | Pride
Proteomics Proteomic analysis of primary human microvascular endothelial cells (HMVECs) stimulated for 24 h with either 50 ï�g/mL recombinant Fibrillin-1 C-terminal fragment or 25 ng/ml VEGF-A compared to unstimulated cells.
2023-05-31 | PXD029834 | Pride
Proteomics Toxoplasma gondii MOB1 interactome
2022-02-16 | PXD028781 | Pride
Proteomics Sirtuin 2 Promotes HCMV Replication by Regulating Cell Cycle Progression
2023-10-13 | PXD041171 | Pride
Proteomics Analysis of the impact of DICER1 and DROSHA on the angio-genic capacity of human endothelial cells
2022-02-17 | PXD027923 | Pride
Transcriptomics OncomiR-21 triggers growth arrest of endothelial cells via a complex network involving p21 CDK2 CDC25A and NFIB
2013-01-17 | E-MEXP-3596 | biostudies-arrayexpress
Proteomics Proteomic analysis of chemical and genetic EZH2 inhibition in diffuse intrinsic pontine glioma cell lines
2022-04-01 | PXD017525 | Pride
Proteomics Proteomic profiling of centrosomes across multiple cell and tissue types by a new affinity capture method
2023-09-18 | PXD043906 | Pride
Proteomics Acid sphingomyelinase deactivation post-ischemia/ reperfusion promotes cerebral angiogenesis and brain remodeling via small extracellular vesiclesAcid sphingomyelinase deactivation post-ischemia/ reperfusion promotes cerebral angiogenesis and brain remodeling via small extracellular vesicles
2022-10-14 | PXD024106 | Pride
Transcriptomics Gene expression profile after CCM1 over expression in human umbilical vein endothelial cells
2010-06-08 | GSE18014 | GEO