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PCGF1-PRC1 links chromatin repression with DNA replication during hematopoietic cell lineage commitment

ABSTRACT: Polycomb repressive complexes 1 and 2 (PRC1 and 2) repress lineage inappropriate genes during development to maintain proper cellular identities. To reveal the function of a variant PRC1 containing PCGF1 (PCGF1-PRC1), we prepared PCGF1 interacting proteins by immunoprecipitation and characterized them by LC-MS/MS.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Bone Marrow

SUBMITTER: Yong-Woon Han

LAB HEAD: Haruhiko Koseki

PROVIDER: PXD036330 | Pride | 2022-11-30

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	001_NC1_100ng_T120SW_B3.wiff	Wiff
	001_NC1_100ng_T120SW_B3.wiff.scan	Wiff
	001_WT1_250ng_T120SW_B3.wiff	Wiff
	001_WT1_250ng_T120SW_B3.wiff.scan	Wiff
	002_NC2_100ng_T120SW_B3.wiff	Wiff

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Publications

PCGF1-PRC1 links chromatin repression with DNA replication during hematopoietic cell lineage commitment.

Takano Junichiro J Ito Shinsuke S Dong Yixing Y Sharif Jafar J Nakajima-Takagi Yaeko Y Umeyama Taichi T Han Yong-Woon YW Isono Kyoichi K Kondo Takashi T Iizuka Yusuke Y Miyai Tomohiro T Koseki Yoko Y Ikegaya Mika M Sakihara Mizuki M Nakayama Manabu M Ohara Osamu O Hasegawa Yoshinori Y Hashimoto Kosuke K Arner Erik E Klose Robert J RJ Iwama Atsushi A Koseki Haruhiko H Ikawa Tomokatsu T

Nature communications 20221128 1

Polycomb group proteins (PcG), polycomb repressive complexes 1 and 2 (PRC1 and 2), repress lineage inappropriate genes during development to maintain proper cellular identities. It has been recognized that PRC1 localizes at the replication fork, however, the precise functions of PRC1 during DNA replication are elusive. Here, we reveal that a variant PRC1 containing PCGF1 (PCGF1-PRC1) prevents overloading of activators and chromatin remodeling factors on nascent DNA and thereby mediates proper de ...[more]

PMID: 36443290

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2024-02-14 | PXD040888 | Pride

RNA polymerase II promotes the organization of chromatin following DNA replication

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2024-02-14 | PXD046514 | Pride

RNA polymerase II acts to promote the organization of chromatin following DNA replication

Project description:In mammals, following the passage of the replisome, nucleosomes lose their defined positioning and transcription contributes to their re-organisation. However, whether transcription plays a greater role in the organization of chromatin following DNA replication remains unclear. By performing iPOND-TMT time course experiments in the presents of transcription inhibition, we show that RNAPII promotes the re-association of hundreds of proteins with newly replicated DNA (Data uploaded under PXD046514). To show that this function of RNAPII is specific to newly replicated chromatin, an iPOND-TMT 24hr chase experiment was performed in the presence of transcription inhibition to examine its effect on the protein binding on steady state chromatin.

2024-02-14 | PXD046546 | Pride

Proteomic profiling reveals distinct phases to the restoration of chromatin following DNA replication

Project description:Chromatin organization must be maintained during cell proliferation to preserve cellular identity and genome integrity. However, DNA replication results in transient displacement of DNA bound proteins, and it is unclear how they regain access to newly replicated DNA. Using quantitative MS-based proteomics coupled to isolation of proteins on nascent DNA (iPOND), we provide time resolved binding kinetics for thousands of proteins behind replisomes in the lung fibroblast cell line TIG-3 (JCRB0506, JCRB Cell Bank). Performing hierarchical clustering, we identified four groups of protein behaviour: Transient enriched on nascent, restored within 11min, peaking in G2/M, and delayed restored. Overall, our data show that most proteins (85%) regain access within the first 2h after the passage of the fork. Only a small percentage of proteins is restored after 2h post-replication, with 5.3 % of factors peaking in G2/M and 9.1% factors not restored before the next G1 phase. We provide evidence that DNA replication not only disrupts but also promotes recruitment of transcription factors and chromatin remodelers, providing a significant advance in understanding how DNA replication could contribute to programmed changes of cell memory.

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Dynamics of chromatin maturation after genome replication

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