Proteomics

Dataset Information

0

Senolytics improve the neurogenic potential of non-glial progenitors and neurorepair in the dorsal pallium of aged killifish independent of inflammation


ABSTRACT: With advancing age, senescent cells accumulate as they are not efficiently cleared by the immune system anymore. Via a senescence-associated secretory phenotype, chronic senescent cells alter the microenvironment, creating an unfavorable milieu for neurogenesis and neurorepair. Using an innovative and rapid aging model, the African turquoise killifish, we have previously demonstrated a dramatic decline in neurogenic potential of non-glial progenitors with age. Even after traumatic brain injury, progenitor proliferation and neuron production was very low in aged killifish in comparison to young adult killifish, and overall neurorepair was incomplete. In the present study, we validated if the senolytic cocktail dasatinib and quercetin (D+Q) could reboot the neurogenic output by clearing chronic senescent cells from the aged killifish brain to re-create the necessary supportive environment. Our results confirm that the aged killifish telencephalon holds a very high senescent cell burden, which we could diminish by short-term systemic D+Q treatment. As a consequence of D+Q administration, proliferation of non-glial progenitors increased and more new neurons were generated and migrated into the parenchyma after injury. Injury-induced inflammation and glial scarring, a phenomenon only seen in aged killifish, remained unaltered. Senolytic treatment with D+Q might thus hold promise for improving brain function in aged populations, and is especially interesting for reviving the neurogenic potential of an already aged central nervous system.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Nothobranchius Furzeri

TISSUE(S): Brain

SUBMITTER: Kurt Boonen  

LAB HEAD: Lut Arckens

PROVIDER: PXD036437 | Pride | 2023-07-20

REPOSITORIES: pride

Dataset's files

Source:
Action DRS
EliseB2.raw Raw
EliseB3.raw Raw
EliseB4.raw Raw
EliseB5.raw Raw
EliseB6.raw Raw
Items per page:
1 - 5 of 17
altmetric image

Publications

A short dasatinib and quercetin treatment is sufficient to reinstate potent adult neuroregenesis in the aged killifish.

Van Houcke Jolien J   Mariën Valerie V   Zandecki Caroline C   Ayana Rajagopal R   Pepermans Elise E   Boonen Kurt K   Seuntjens Eve E   Baggerman Geert G   Arckens Lutgarde L  

NPJ Regenerative medicine 20230616 1


The young African turquoise killifish has a high regenerative capacity, but loses it with advancing age, adopting several aspects of the limited form of mammalian regeneration. We deployed a proteomic strategy to identify pathways that underpin the loss of regenerative power caused by aging. Cellular senescence stood out as a potential brake on successful neurorepair. We applied the senolytic cocktail Dasatinib and Quercetin (D + Q) to test clearance of chronic senescent cells from the aged kill  ...[more]

Similar Datasets

2024-01-09 | GSE252835 | GEO
2023-12-08 | GSE243616 | GEO
2023-09-26 | PXD031782 | Pride
2021-02-06 | GSE161340 | GEO
2023-05-22 | GSE228941 | GEO
2021-10-22 | GSE155596 | GEO
2020-05-01 | GSE135406 | GEO
2022-09-06 | GSE210020 | GEO
2023-07-03 | PXD041973 | Pride
2023-07-03 | PXD041777 | Pride