Dataset Information

Stability of the neuronal fate determinant MEIS2 is controlled by calpain2

ABSTRACT: Calpains are non-lysosomal, Ca2+-dependent cysteine proteases, which are associated with various cellular functions but have so far been mainly studied in the context of disease. Their contribution to homeostasis in the healthy organism is still not well understood and their substrates have remained enigmatic in most cases. In the present study, we describe a previously unrecognized role for the calpain protease calpain2 in the regulation of neuronal differentiation of adult neural stem- and progenitor cells through cleavage and elimintation of the neuronal fate determinant MEIS2. Mass spectrometry analysis was performed on immunoprecipitated MEIS2 protein to identify phosphory¬lated residues in MEIS2 and on immunoprecipitated MEIS2 incubated with native porcine calpain2 to map calpain2-induced cleavage sites in the protein.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human) Mus Musculus (mouse)

TISSUE(S): Cell Culture

SUBMITTER: Ilka Wittig

LAB HEAD: Dorothea Schulte

PROVIDER: PXD036442 | Pride | 2024-02-08

REPOSITORIES: Pride

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The neuronal transcription factor MEIS2 is a calpain-2 protease target.

Müller Tanja T Müller Tanja T Reichlmeir Marina M Hau Ann-Christin AC Wittig Ilka I Schulte Dorothea D

Journal of cell science 20240228 4

Tight control over transcription factor activity is necessary for a sensible balance between cellular proliferation and differentiation in the embryo and during tissue homeostasis by adult stem cells, but mechanistic details have remained incomplete. The homeodomain transcription factor MEIS2 is an important regulator of neurogenesis in the ventricular-subventricular zone (V-SVZ) adult stem cell niche in mice. We here identify MEIS2 as direct target of the intracellular protease calpain-2 (compo ...[more]

PMID: 38305737

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			Action	DRS
	P18_087_Meis_IP_B.mgf	Mgf
	P18_087_Meis_IP_B.raw	Raw
	P18_087_Meis_IP_C.mgf	Mgf
	P18_087_Meis_IP_C.raw	Raw
	P18_087_Meis_IP_D.mgf	Mgf