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Trans-synaptic dwelling of SARS-CoV-2 particles perturbs neural synapse organization and function

ABSTRACT: we performed mass spectrometry-based differential proteomics on single cortical organoids infected in the presence or absence of SARS-CoV-2 to identify the molecular perturbations caused by the virus.

INSTRUMENT(S): Q Exactive HF-X

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Brain

SUBMITTER: Francois Delalande

LAB HEAD: Christine Carapito

PROVIDER: PXD036485 | Pride | 2024-02-16

REPOSITORIES: pride

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			Action	DRS
	F091744.dat	Other
	F091749.dat	Other
	F091750.dat	Other
	F091751.dat	Other
	F091752.dat	Other

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ASTROGLIOSIS AND NEUROINFLAMMATION UNDERLIE SCOLIOSIS UPON CILIA DYSFUNCTION

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16SrRNA analysis from wildtype mice that were either fed or not with low protein diets for 4 weeks

Project description:We found that low protein diet consumption resulted in decrease in the percentage of normal Paneth cell population in wild type mice, indicating that low protein diet could negatively affect Paneth cell function. We performed fecal microbiota composition profiling. Male mice were used at 4-5 weeks of age. Fecal samples were collected for microbiome analysis.

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Changes in intestinal permeability and gut microbiota following diet-induced weight loss in patients with metabolic dysfunction-associated steatohepatitis and liver fibrosis

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N2-methylguanosine modifications on human tRNAs and snRNA U6 are important for colon cancer cell proliferation, protein translation and pre-mRNA splicing

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ProMetIS: deep phenotyping of mouse models by proteomics and metabolomics.

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A longer isoform of Stim1 is a negative SOCE regulator but increases cAMP modulated NFAT signaling

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16S rRNA analysis from wild type mice that were either fed or not with western diet for 3 generations

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16S rRNA sequencing of fecal samples from Atg16l1 T300A and wildtype littermate mice that were exposed to mainstream cigarette smoking co-housed with mice from the same genotype but not exposed to cigarette smoking

Project description:We found that mainstream cigarette smoking (4 cigarettes/day, 5 days/week for 2 weeks using Kentucky Research Cigarettes 3R4F) resulted in >20% decrease in the percentage of normal Paneth cell population in Atg16l1 T300A mice but showed minimal effect in wildtype littermate control mice, indicating that Atg16l1 T300A polymorphism confers sensitivity to cigarette smoking-induced Paneth cell damage. We performed cohousing experiments to test if Paneth cell phenotype is horizontally transmissible as is microbiota. Atg16l1 T300A and littermate controls that were exposed to cigarette smoking were used as microbiota donors, and these donor mice were exposed to smoking for 2 weeks prior to cohousing. Separate groups of Atg16l1 T300A and littermate controls that were not exposed to cigarette smoking were used as microbiota recipients. The microbiota recipients were co-housed with microbiota donors of the same genotype for 4 weeks, during this period the donors continued to be exposed to cigarette smoking. Cigarette smoking was performed using smoking chamber with the dosage and schedule as described above. At the end of the experiment, the fecal microbiota composition was analyzed by 16S rRNA sequencing.

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