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The inhibition of putrescine synthesis affects the in vitro shoot development of Cedrela fissilis Vell. (Meliaceae) by altering endogenous polyamine metabolism and the proteomic profile

ABSTRACT: Polyamines (PAs) and proteins have been demonstrated to be fundamental for in vitro shoot development of Cedrela fissilis. We evaluated the influence of 6-benzyladenine (BA) and putrescine (Put) on the growth of shoots, PA metabolism and proteomic profiles of C. fissilis. The longest shoots were obtained under 2.5 μM BA + 2.5 mM Put treatment. The inhibition of Put synthesis by D-arginine (D-arg) decreased the shoot length and reduced the endogenous contents of free Put and the activities of the Put biosynthesis enzymes arginine decarboxylase (ADC) and ornithine decarboxylase (ODC), resulting in reduced shoot growth. The ODC activity was higher than that of ADC, which is the main enzyme in the synthesis of Put in C. fissilis. Inhibition of Put synthesis affected the proteomic profile, reducing the accumulation of the ubiquitin receptor RAD23c, peroxidase 15, ADP-ribosylation factor 1, ADP-ribosylation factor-like protein 8a, profilin-4, profilin-2, glucan endo-1,3-beta-glucosidase, and expansin-like B1 and increasing the accumulation of V-type proton ATPase catalytic subunit A and methionine gamma-lyase, highlighting the relevance of these proteins in increasing shoot length. The transport protein SEC13 homolog B and the basic isoform glucan endo-1,3-beta-glucosidase, which are unique to shoots treated with BA+Put, are related to the promotion of shoot growth. Our results show that the modulation of endogenous PAs and proteomic profiles is necessary to regulate in vitro morphogenesis in C. fissilis. Moreover, the ODC enzyme is highly involved in the synthesis of Put during in vitro shoot development and is described for the first time in this species.

INSTRUMENT(S): SYNAPT G2-Si

ORGANISM(S): Cedrela Fissilis

TISSUE(S): Plant Cell, Leaf, Shoot

SUBMITTER: Claudete Santa-Catarina

LAB HEAD: Claudete Santa Catarina

PROVIDER: PXD036504 | Pride | 2023-09-06

REPOSITORIES: Pride

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			Action	DRS
	2019_10_17_1Darg_Victor_001.raw.zip	Raw
	2019_10_17_1Darg_Victor_002.raw.zip	Raw
	2019_10_17_1Darg_Victor_003.raw.zip	Raw
	2019_10_17_2Darg_Victor_004.raw.zip	Raw
	2019_10_17_2Darg_Victor_005.raw.zip	Raw

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The inhibition of putrescine synthesis affects the in vitro shoot development of Cedrela fissilis Vell. (Meliaceae) by altering endogenous polyamine metabolism and the proteomic profile

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