Proteomics

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Isobaric labeling-based quantitative proteomics of cells purified from the intestine of Crohn’s disease patients reveals immune cell-epithelial cell crosstalk of potential importance in disease pathogenesis


ABSTRACT: Crohn's disease (CD) is a chronic condition characterized by recurrent flares of inflammation of the gastrointestinal tract. Despite decades of research, the etiology of CD is poorly understood and is characterized by dysregulated immune activation that progressively destroys the gastrointestinal tissue. At the center of the pathology is the intestinal mucosa, where the epithelium and the lamina propria are main cellular compartments. While the epithelium contains predominantly epithelial cells, the lamina propria is enriched in immune cells and contains supporting stroma. Thus, the cells constituting these compartments can be classified as epithelial cells, immune cells and stromal cells. Gaining insight into how these cell types interact with each other is important to further our understanding of CD pathogenesis. Here, using isobaric labeling-based quantitative proteomics, we perform an exploratory study to analyze in-depth proteome changes in epithelial cells, immune cells and stromal cells purified by cell sorting from the intestinal mucosa of CD patients and controls. Our workflow preserving the link between the different cell types in individuals opens the possibility to explore cellular crosstalk and to further refine data on cell-cell interactions in the development of CD.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Small Intestine Mucosa

DISEASE(S): Crohn's Disease

SUBMITTER: Ivo Fabrik  

LAB HEAD: Mary Jo Wick

PROVIDER: PXD036591 | Pride | 2023-01-02

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
4083_SPS65_IAA_Lumos_201009_set1_2_3.pdResult Other
Lumos_201009_19.raw Raw
Lumos_201009_20.raw Raw
Lumos_201009_21.raw Raw
Lumos_201009_22.raw Raw
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