Dataset Information

Integrated omic analysis reveals multilevel control of renal and systemic metabolism by the renal tubular circadian clock

ABSTRACT: Circadian rhythmicity in renal function suggests a requirement for circadian adaptations in renal metabolism. We studied circadian changes in renal metabolic pathways using integrated transcriptomic, proteomic and metabolomic analysis performed on control mice and mice deficient in the circadian clock gene Bmal1 in the renal tubule (cKOt mice). Proteins were extracted from whole kidneys of 60 mice. Of these, 30 were conditional knockouts of Arntl (Bmal1) and 30 were of control genotype. They were housed under 12-hours light/12-hours dark cycles and were sacrificed at six different time points: zeitgeber time ZT 0, ZT 4, ZT 8, ZT 12, ZT 16, ZT 20 ( ZT 0 being the time of light on and ZT 12 the time of light off). Five replicates per genotype and time point were analysed.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Kidney

SUBMITTER: Manfredo Quadroni

LAB HEAD: Dmitri Firsov

PROVIDER: PXD036803 | Pride | 2023-03-15

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	200916_Firsov_12446B-SCX-075.raw	Raw
	200916_Firsov_12446B-SCX-150.raw	Raw
	200916_Firsov_12446B-SCX-NH3.raw	Raw
	200917_Firsov_12447B-SCX-075.raw	Raw
	200917_Firsov_12447B-SCX-150.raw	Raw

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Publications

Multiomics reveals multilevel control of renal and systemic metabolism by the renal tubular circadian clock.

Bignon Yohan Y Wigger Leonore L Ansermet Camille C Weger Benjamin D BD Lagarrigue Sylviane S Centeno Gabriel G Durussel Fanny F Götz Lou L Ibberson Mark M Pradervand Sylvain S Quadroni Manfredo M Weger Meltem M Amati Francesca F Gachon Frédéric F Firsov Dmitri D

The Journal of clinical investigation 20230417 8

Circadian rhythmicity in renal function suggests rhythmic adaptations in renal metabolism. To decipher the role of the circadian clock in renal metabolism, we studied diurnal changes in renal metabolic pathways using integrated transcriptomic, proteomic, and metabolomic analysis performed on control mice and mice with an inducible deletion of the circadian clock regulator Bmal1 in the renal tubule (cKOt). With this unique resource, we demonstrated that approximately 30% of RNAs, approximately 20 ...[more]

PMID: 36862511

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