Proteomics

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Quantitative proteomic profiling of androgenic sexual endocrine perturbation in zebrafish embryos


ABSTRACT: Endocrine disruption (ED) can trigger far-reaching effects on environmental populations, justifying a refusal of market approval for chemicals with ED properties. For the assessment of ED effects on development and reproduction, regulatory decisions mostly rely on apical endpoints from in vivo testing with adult animals. Here, we present a rapid and reproducible data dependent proteomics approach for identifying comprehensive molecular fingerprints interfering with the sexual endocrine system in zebrafish (Danio rerio) embryos as an alternative to animal testing. For this, we have analysed methyltestosterone as model substances for androgenic perturbation in a modified zebrafish embryo toxicity test (zFET). These signatures allow for a definition of solid biomarkers as tools in screening approaches and for integration in chronic toxicity studies for identifying suspect ED substances, in the fish early life-stage test (OECD TG 210).

INSTRUMENT(S): Q Exactive

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Embryo

SUBMITTER: Steve Ayobahan  

LAB HEAD: Dr. Sebastian Eilebrecht

PROVIDER: PXD036875 | Pride | 2023-02-25

REPOSITORIES: Pride

Dataset's files

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Action DRS
TMT_81_001.raw Raw
TMT_81_002.raw Raw
TMT_81_003.raw Raw
TMT_82_001.raw Raw
TMT_82_002.raw Raw
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Publications

Comprehensive identification of gene expression fingerprints and biomarkers of sexual endocrine disruption in zebrafish embryo.

Ayobahan Steve U SU   Alvincz Julia J   Reinwald Hannes H   Strompen Jannis J   Salinas Gabriela G   Schäfers Christoph C   Eilebrecht Elke E   Eilebrecht Sebastian S  

Ecotoxicology and environmental safety 20230104


Endocrine disruptors (EDs), capable of modulating the sex hormone system of an organism, can exert long-lasting negative effects on reproduction in both humans and the environment. For these reasons, the properties of EDs prevent a substance from being approved for marketing. However, regulatory testing to evaluate endocrine disruption is time-consuming, costly, and animal-intensive. Here, we combined sublethal zebrafish embryo assays with transcriptomics and proteomics for well-characterized en  ...[more]

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