Ontology highlight
ABSTRACT:
INSTRUMENT(S): Q Exactive
ORGANISM(S): Homo Sapiens (human)
SUBMITTER: Vincent Spegg
LAB HEAD: Prof. Dr. Matthias Altmeyer
PROVIDER: PXD036935 | Pride | 2023-03-15
REPOSITORIES: Pride
Items per page: 1 - 5 of 23 |
Nature structural & molecular biology 20230309 4
RPA has been shown to protect single-stranded DNA (ssDNA) intermediates from instability and breakage. RPA binds ssDNA with sub-nanomolar affinity, yet dynamic turnover is required for downstream ssDNA transactions. How ultrahigh-affinity binding and dynamic turnover are achieved simultaneously is not well understood. Here we reveal that RPA has a strong propensity to assemble into dynamic condensates. In solution, purified RPA phase separates into liquid droplets with fusion and surface wetting ...[more]