Identification and functional characterization of proteins interacting with cohesin complex in yeast Saccharomyces cerevisiae.

ABSTRACT: To ensure equal separation of DNA, sister chromatids are held together from S phase to metaphase–anaphase transition by a multiprotein complex called cohesin. This makes it possible to establish chromosome biorientation, counteracts the pulling force of mitotic spindle microtubules, preventing premature sister chromatid separation, and ensures precise segregation of sister DNAs into daughter cells In order to better understand how the sister chromatid cohesion process is regulated we looked for new cohesin interacors. We constructed a yeast strain endogenously expressing TAP-tagged Scc1 (Scc1-TAP). Next, we performed a single-step TAP purification using an untagged strain (mock sample) or a strain expressing Scc1-TAP, followed by identification of co-purifying proteins by MS.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Enterococcus Faecalis (streptococcus Faecalis) Heliocidaris Erythrogramma (sea Urchin) Bombyx Mori (silk Moth) Pan Troglodytes (chimpanzee) Candida Albicans (yeast) Pinus Radiata (monterey Pine) Brassica Oleracea Var. Botrytis (cauliflower) Helicobacter Pylori J99 (campylobacter Pylori J99) Taeniopygia Guttata (zebra Finch) (poephila Guttata) Saccharomyces Cerevisiae (baker's Yeast) Merluccius Bilinearis (silver Hake) Halobacterium Salinarum Sus Scrofa Domesticus (domestic Pig) Drosophila Melanogaster (fruit Fly) Hepatitis C Virus Subtype 1a Pongo Pygmaeus (bornean Orangutan) Escherichia Coli Struthio Camelus (ostrich) Ictalurus Punctatus (channel Catfish) Hordeum Vulgare (barley) Brassica Napus (rape) Staphylococcus Aureus Ricinus Communis Dictyostelium Discoideum (slime Mold) Olea Europaea (common Olive) Gadus Morhua (atlantic Cod) Rattus Norvegicus (rat) Equus Caballus (horse) Pisum Sativum (garden Pea) Caenorhabditis Elegans Danio Rerio (zebrafish) (brachydanio Rerio) Solanum Tuberosum (potato) Oryza Sativa (rice) Rattus Rattus (black Rat) Macaca Mulatta (rhesus Macaque) Mesocricetus Auratus (golden Hamster) Nicotiana Tabacum (common Tobacco) Cercopithecus Aethiops (green Monkey) (grivet) Homo Sapiens (human) Bos Taurus (bovine) Gallus Gallus (chicken) Cicer Arietinum (chickpea) (garbanzo) Mus Musculus (mouse)

SUBMITTER: Agata Malinowska  

LAB HEAD: Ireneusz Litwin

PROVIDER: PXD037034 | Pride | 2023-10-24

REPOSITORIES: Pride