Proteomics

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MASP micro-specimen samples at different spatial locations

ABSTRACT: Quantitative protein mapping on whole-tissue levels provides important insights into the spatially-organized regulatory processes/networks related to diseases and therapy, but remains a tremendous challenge. We describe a micro-scaffold assisted spatial proteomics(MASP) method, based on precise tissue spatial-compartmentalization using a 3D-printed micro-scaffold, capable of mapping thousands of proteins across a whole-tissue slice with excellent quantitative quality. The mapping accuracy was validated and applied in mapping >5,000 cerebral proteins in mouse brain. Under stringent cutoffs, 5019 unique proteins were mapped(N=208 micro-specimens) and 4577 proteins were mapped in all regions.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Brain

SUBMITTER: Shihan Huo  

LAB HEAD: Jun Qu

PROVIDER: PXD037041 | Pride | 2023-03-11

REPOSITORIES: Pride

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MASP_A1_180min_240k_clm163_65cm_HCD_OT_val50_HCD30.raw Raw
MASP_A1_180min_240k_clm163_65cm_HCD_OT_val50_HCD30.raw.mzXML Mzxml
MASP_A2_180min_240k_clm163_65cm_HCD_OT_val50_HCD30.raw Raw
MASP_A2_180min_240k_clm163_65cm_HCD_OT_val50_HCD30.raw.mzXML Mzxml
MASP_A3_180min_240k_clm163_65cm_HCD_OT_val50_HCD30.raw Raw
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Publications

In-depth mapping of protein localizations in whole tissue by micro-scaffold assisted spatial proteomics (MASP).

Ma Min M   Huo Shihan S   Zhang Ming M   Qian Shuo S   Zhu Xiaoyu X   Pu Jie J   Rasam Sailee S   Xue Chao C   Shen Shichen S   An Bo B   Wang Jianmin J   Qu Jun J  

Nature communications 20221214 1

Accurate, in-depth mapping of proteins on whole-tissue levels provides comprehensive insights into the spatially-organized regulatory processes/networks in tissues, but is challenging. Here we describe a micro-scaffold assisted spatial proteomics (MASP) strategy, based on spatially-resolved micro-compartmentalization of tissue using a 3D-printed micro-scaffold, capable of mapping thousands of proteins across a whole-tissue slice with excellent quantitative accuracy/precision. The pipeline includ  ...[more]

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