Proteomics

Dataset Information

0

The Legionella protein VpdC is a ubiquitin-activated phospholipase effector


ABSTRACT: The goal of this project was to identify the host factor(s) required for allosteric activation of the phospholipase VpdC from the intracellular pathogen Legionella pneumophila. For that reason, we performed co-precipitation analyses from lysate of transiently transfected HEK293T cells producing either Red Fluorescent Protein (RFP)-tagged VpdC(257-884) or RFP-VpdC(257-610) as bait.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Legionella Pneumophila

TISSUE(S): Cell Culture, Kidney

SUBMITTER: David Anderson  

LAB HEAD: Matthias Peter Machner

PROVIDER: PXD037059 | Pride | 2023-06-12

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
allSpectra.CID.ITMS.iso_0.apl Other
allSpectra.CID.ITMS.iso_1.apl Other
allSpectra.CID.ITMS.iso_10.apl Other
allSpectra.CID.ITMS.iso_11.apl Other
allSpectra.CID.ITMS.iso_12.apl Other
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Publications

VpdC is a ubiquitin-activated phospholipase effector that regulates <i>Legionella</i> vacuole expansion during infection.

Li Xiao X   Anderson D Eric DE   Chang Yuen-Yan YY   Jarnik Michal M   Machner Matthias P MP  

Proceedings of the National Academy of Sciences of the United States of America 20221121 48


Intravacuolar pathogens need to gradually expand their surrounding vacuole to accommodate the growing number of bacterial offspring during intracellular replication. Here we found that <i>Legionella pneumophila</i> controls vacuole expansion by fine-tuning the generation of lysophospholipids within the vacuolar membrane. Upon allosteric activation by binding to host ubiquitin, the type IVB (Dot/Icm) effector VpdC converts phospholipids into lysophospholipids which, at moderate concentrations, ar  ...[more]

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