Dataset Information

Enzymatic investigation of Spongospora subterranea zoospore attachment to roots of potato cultivars resistant or susceptible to powdery scab disease

ABSTRACT: For potato crops, host resistance is currently the most effective and sustainable tool to manage potato root and tuber diseases caused by the plasmodiophorid, Spongospora subterranea. Arguably, zoospore root attachment is the most critical phase of the pathogen infection, however, the mechanisms underlying zoospore root attachment remains unknown. This study investigated the potential role of root cell wall surface polysaccharides and proteins in zoospore root attachment in resistant and susceptible potato cultivars. We first compared the effects of enzymatic removal of root cell wall proteins, N-linked glycans or polysaccharides on S. subterranea attachment to root tissue of resistant and susceptible potato cultivars. Subsequently, mass spectrometry analysis of peptides released by trypsin shaving (TS) of root segments identified 1235 proteins, of which 262 were differentially abundant between the resistant and susceptible cultivars. In particular, proteins associated with glutathione metabolism and lignin biosynthesis were more abundant in the resistant cultivar. Comparison with whole-root proteomic analysis of the same resistant and susceptible cultivars led to identification of 226 proteins unique to the TS dataset, of which 188 were significantly different between cultivars. Among these, the pathogen defence-related cell wall protein stem 28 kDa glycoprotein and two major latex proteins were significantly less abundant in the resistant cultivar compared to the susceptible cultivar. A further major latex protein was detected at reduced levels in the resistant cultivar in both TS and whole-root proteomic datasets. In contrast, in the TS-specific dataset, three glutathione S-transferase proteins were more abundant in the resistant cultivar, while the protein glucan endo-1,3-beta-glucosidase was significantly increased in both the TS and whole-root datasets. These results imply a particular role of major latex proteins and glucan endo-1,3-beta-glucosidase in the regulation of host susceptibility to S. subterranea.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Solanum Tuberosum (potato)

TISSUE(S): Root

SUBMITTER: Richard Wilson

LAB HEAD: Dr Richard Wilson

PROVIDER: PXD037060 | Pride | 2023-03-11

REPOSITORIES: Pride

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Enzymatic Investigation of <i>Spongospora subterranea</i> Zoospore Attachment to Roots of Potato Cultivars Resistant or Susceptible to Powdery Scab Disease.

Yu Xian X Wilson Richard R Eyles Alieta A Balotf Sadegh S Tegg Robert Stephen RS Wilson Calum Rae CR

Proteomes 20230209 1

For potato crops, host resistance is currently the most effective and sustainable tool to manage diseases caused by the plasmodiophorid <i>Spongospora subterranea</i>. Arguably, zoospore root attachment is the most critical phase of infection; however, the underlying mechanisms remain unknown. This study investigated the potential role of root-surface cell-wall polysaccharides and proteins in cultivars resistant/susceptible to zoospore attachment. We first compared the effects of enzymatic remov ...[more]

PMID: 36810563

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Project description:Background: Cylindrocladium parasiticum Crous, Wingfield & Alfenas, the causal agent of Cylindrocladium black rot (CBR) of peanut (Arachis hypogaea L.), has leaded to economic losses in China. Learning about how peanut responds to C. parasiticum infection will be conducive to designing strategies for CBR control. However, the response of peanut plant to C. parasiticum is poorly understood. Results: In this study, two contrasting peanut cultivars, T09 (C. parasiticum-resistant) and P562 (C. parasiticum-susceptible) were used for comparative analysis of protein profiles in the root segment of peanut plants in responses to C. parasiticum infection. Proteomic profiling identified 1647 and 391 differentially expressed proteins (DEPs) in A. hypogaea L. P562 and A. hypogaea L. T09, respectively, compared to controls. A total of 350 and 1095 DEPs were identified between A. hypogaea L. P562 and A. hypogaea L. T09 before and after 9 dpi, respectively. Functional categorization by GO annotation showed that C. parasiticum-responsive proteins were mainly involved in catalytic activity and binding. The results of KEGG pathway analysis indicated both resistant and susceptible peanut cultivars can regulate gene expression in the phenylpropanoid pathway, terpenoid backbone biosynthesis, SA, and JA pathways to induce defensive genes and protein expression which enhances plant defence capacity. However, the MAPK signal pathway was more pronounced in resistant peanut cultivar T09. We also observed an increase of CYP73A100 involved in phenylpropanoid biosynthesis and flavonoid biosynthesis pathways in the susceptible peanut ecotype P562, while decrease in the resistant peanut ecotype T09, after 9 dpi. Additionally, there was a marked activation of brassinosteroid biosynthesis in the resistant T09, which indicated a possible involvement of activation of plant immune response in the resistant responses of peanut to C. parasiticum. Conclusions: This study provides some insights into the molecular networks involved on cellular and physiological responses to C. parasiticum infestation.

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