Proteomics

Dataset Information

0

The Eyes Absent family members EYA4 and EYA1 promote PLK1 activation and successful mitosis through tyrosine dephosphorylation: PLK1 PRM


ABSTRACT: The aim of these experiments was to quantify PLK1 tyrosine phosphosites with respect to EYA4 and EYA1. PRM was performed on immunopurified PLK1. This was part of a larger project to characterize the effects of EYA4 mediated dephosphorylation on PLK1 functions.

INSTRUMENT(S): Q Exactive

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Christopher Nelson  

LAB HEAD: Hilda Amelia

PROVIDER: PXD037064 | Pride | 2024-02-26

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
04032020_Benz_A.msf Msf
04032020_Benz_A.raw Raw
04032020_Benz_B.msf Msf
04032020_Benz_B.raw Raw
04032020_Benz_C.msf Msf
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Publications


The Eyes Absent proteins (EYA1-4) are a biochemically unique group of tyrosine phosphatases known to be tumour-promoting across a range of cancer types. To date, the targets of EYA phosphatase activity remain largely uncharacterised. Here, we identify Polo-like kinase 1 (PLK1) as an interactor and phosphatase substrate of EYA4 and EYA1, with pY445 on PLK1 being the primary target site. Dephosphorylation of pY445 in the G2 phase of the cell cycle is required for centrosome maturation, PLK1 locali  ...[more]

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