Proteomics

Dataset Information

0

Protein interaction partners of FRUITFULL in inflorescence meristems and pistils by IP-MS.

ABSTRACT: To study the possibility that the tissue-specific gene targets and regulation by FUL are due to a different composition in the FUL transcription factor (TF) protein complex/es in both tissues, we determined the protein-protein interactions of FUL in planta. Inflorescence meristems (IMs) of 35S:AP1-GR ap1 cal pFUL:FUL-GFP plants were collected to identify meristem-specific protein complexes of FUL, and stage 12 - 16 pistils of pFUL:FUL-GFP ful-1 plants were collected for the pistil-specific FUL protein complexes. Protein complexes were isolated by immunoprecipitation (IP) using anti-GFP antibodies and protein identification was performed using LC-MS/MS, followed by label-free protein quantification.

INSTRUMENT(S): LTQ Orbitrap XL

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Flower, Inflorescence Meristem

SUBMITTER: Sjef Boeren  

LAB HEAD: Sjef Boeren

PROVIDER: PXD037091 | Pride | 2023-03-30

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
KK362_Col0_ap1cal_1.raw Raw
KK363_Col0_ap1cal_2.raw Raw
KK364_Col0_ap1cal_3.raw Raw
KK365_FUL_1.raw Raw
KK366_FUL_2.raw Raw
Items per page:
1 - 5 of 14
altmetric image

Publications

Dual specificity and target gene selection by the MADS-domain protein FRUITFULL.

van Mourik Hilda H   Chen Peilin P   Smaczniak Cezary C   Boeren Sjef S   Kaufmann Kerstin K   Bemer Marian M   Angenent Gerco C GC   Muino Jose M JM  

Nature plants 20230216 3

How transcription factors attain their target gene specificity and how this specificity may be modulated, acquiring different regulatory functions through the development of plant tissues, is an open question. Here we characterized different regulatory roles of the MADS-domain transcription factor FRUITFULL (FUL) in flower development and mechanisms modulating its activity. We found that the dual role of FUL in regulating floral transition and pistil development is associated with its different  ...[more]

Similar Datasets

Transcriptomics Promiscuous gene expression in human medullary thymic epithelial cell subsets
2013-08-31 | E-GEOD-49625 | biostudies-arrayexpress
Proteomics LZY-interacting proteins in Arabidopsis
2020-01-09 | PXD016219 | Pride
Proteomics Arabidopsis Wdr8 interactors identification
2022-02-17 | PXD026063 | Pride
Proteomics Immunoprecipitation of proteins containing Lys48-linked polyUb chains in wild-type C. elegans at day 5 of adulthood
2021-05-26 | PXD024093 | Pride
Proteomics Immunoprecipitation of proteins containing Lys63-linked polyUb chains in wild-type C. elegans at day 5 of adulthood
2021-05-26 | PXD024045 | Pride
Transcriptomics RNA-immunoprecipitation of mTRAN1-GFP
2023-09-01 | E-MTAB-13060 | biostudies-arrayexpress
Genomics Pregnancy-associated alterations in DNA methylation patterns of mammary epithelial stem cells
2015-01-22 | E-GEOD-54150 | biostudies-arrayexpress
Proteomics Arabidopsis SKD1 Proteom LC-MSMS
2021-06-23 | PXD025028 | Pride
Transcriptomics Genome-wide analysis of alternative splicing evolution among Mus subspecies
2010-02-28 | E-GEOD-18905 | biostudies-arrayexpress
Proteomics Identification of FLS2 interacting proteins by co-immunoprecipitation and shot gun proteomics
2016-01-26 | PXD003189 | Pride