Proteomics

Dataset Information

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HDX-MS of NicA2 and a NicA2 variant with increased activity

ABSTRACT: Here we have used HDX-MS to investigate the change in structure/dynamics in NicA2 and a variant with increased activity (v321) that was generated using directed evolution

INSTRUMENT(S): Synapt MS

ORGANISM(S): Homo Sapiens (human)

SUBMITTER: Antonio Calabrese  

LAB HEAD: Antonio Calabrese

PROVIDER: PXD037151 | Pride | 2023-11-16

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
NicA2_NEW.DnX Other
fbsaca_220908_NicA2_01.raw.zip Raw
fbsaca_220908_NicA2_03.raw.zip Raw
fbsaca_220908_NicA2_05.raw.zip Raw
fbsaca_220908_NicA2_07.raw.zip Raw
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Publications

The flavoenzyme nicotine oxidoreductase (NicA2) is a promising injectable treatment to aid in the cessation of smoking, a behavior responsible for one in ten deaths worldwide. NicA2 acts by degrading nicotine in the bloodstream before it reaches the brain. Clinical use of NicA2 is limited by its poor catalytic activity in the absence of its natural electron acceptor CycN. Without CycN, NicA2 is instead oxidized slowly by dioxygen (O<sub>2</sub>), necessitating unfeasibly large doses in a therape  ...[more]

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