Dataset Information

TrxA-binding proteins of Salmonella

ABSTRACT: To identify binding partners of Salmonella’s thioredoxin protein encoded by the trxA gene, we performed tandem-affinity purification (TAP) using a C-terminal fusion of thioredoxin with calmodulin-binding peptide and Protein A. TrxA-binding molecules were identified by mass spectrometry (MS) analysis

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Salmonella Sp. Zz-4

SUBMITTER: Monika Dzieciatkowska

LAB HEAD: Kim, Ju Sim

PROVIDER: PXD037199 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

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			Action	DRS
	QE_20160629_JKim_sample_01.raw	Raw
	QE_20160629_JKim_sample_01__F086000_.mzid.gz	Mzid
	QE_20160629_JKim_sample_01__F086000_.mzid_QE_20160629_JKim_sample_01__F086000_.MGF	Mzid
	QE_20160629_JKim_sample_02.raw	Raw
	QE_20160629_JKim_sample_02__F085999_.mzid.gz	Mzid

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Publications

Promiscuity of response regulators for thioredoxin steers bacterial virulence.

Kim Ju-Sim JS Born Alexandra A Till James Karl A JKA Liu Lin L Kant Sashi S Henen Morkos A MA Vögeli Beat B Vázquez-Torres Andrés A

Nature communications 20221020 1

The exquisite specificity between a sensor kinase and its cognate response regulator ensures faithful partner selectivity within two-component pairs concurrently firing in a single bacterium, minimizing crosstalk with other members of this conserved family of paralogous proteins. We show that conserved hydrophobic and charged residues on the surface of thioredoxin serve as a docking station for structurally diverse response regulators. Using the OmpR protein, we identify residues in the flexible ...[more]

PMID: 36266276

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