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A gephyrin-like protein links cell division and polar elongation in Corynebacteriales

ABSTRACT: To discover missing players in corynebacterial cell division we used mass-spectrometry based interactomics using formaldehyde as crosslinker, starting with the FtsZ membrane anchor SepF as a bait for co-immunoprecipitation (co-IP) of divisome members. For SepF interactome we carried out the co-IPs using anti-Scarlet antibodies in Cglu strains expressing either SepF-mScarlet or mScarlet. Co-IPs using anti-SepF were also performed from Cglu or SepF-mScarlet strains. GLP interactomes were analysed in two strains: Cglu and Cglu_Dglpr strains.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Corynebacterium Glutamicum Atcc 13032

SUBMITTER: Alejandro Leyva

LAB HEAD: Rosario Durán

PROVIDER: PXD037255 | Pride | 2023-07-26

REPOSITORIES: Pride

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			Action	DRS
	210915_Cglutamicum_constructions.fasta	Fasta
	211117_Corynebacterium_glutamicum_ATCC13032.fasta	Fasta
	Cglu-antiGLP_R1.raw	Raw
	Cglu-antiGLP_R1.txt	Txt
	Cglu-antiGLP_R2.raw	Raw

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