Proteomics

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PRM-based quantitative proteomic analysis of ribosomes in GC-1 cell line

ABSTRACT: Relative protein quantification of RPL4 and RPL39L in non-ribosome, 40S, 60S and ribosome fractions by PRM;Absolute quantification of RPL39 and RPL39L levels in ribosomes of GC-1 wild type cell, and RPL39L-IRES-eGFP-overexpressed RibosomeCore-/Y GC-1 cells with or without dox-induced expression of RPL39 by PRM. Bovine serum albumin (BSA) as a negative control.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: yue wang  

LAB HEAD: Xuejiang Guo

PROVIDER: PXD037257 | Pride | 2022-10-19

REPOSITORIES: Pride

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Dataset's files

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GC-1-Ribosome-WT-Lenti-Dox-RPL39_39L-20211228.zip Other
PRM_RPL39_39L_5fmol_500ng_GC-1_Lenti-Dox_Ribo_rep1_20211227.raw Raw
PRM_RPL39_39L_5fmol_500ng_GC-1_Lenti-Dox_Ribo_rep2_20211227.raw Raw
PRM_RPL39_39L_5fmol_500ng_GC-1_Lenti-Dox_Ribo_rep3_20211227.raw Raw
PRM_RPL39_39L_5fmol_500ng_GC-1_Lenti_Dox_Ribo_rep1_20211227.raw Raw
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Publications

Ribosomes are highly sophisticated translation machines that have been demonstrated to be heterogeneous in the regulation of protein synthesis<sup>1,2</sup>. Male germ cell development involves complex translational regulation during sperm formation<sup>3</sup>. However, it remains unclear whether translation during sperm formation is performed by a specific ribosome. Here we report a ribosome with a specialized nascent polypeptide exit tunnel, Ribosome<sup>ST</sup>, that is assembled with the m  ...[more]

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