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Oxidized thioredoxin-1 restrains the NLRP1 inflammasome

ABSTRACT: To find cellular protein binding partners of the NLRP1 inflammasome, FLAG-tagged full length NLRP1 (or a GFP-FLAG protein control) was ectopically expressed in HEK 293T cells and affinity purified from cell lysates (Note: the NLRP1-FLAG-containing lysate samples were affinity purified in the presence or absence of excess proline) before proteins were reduced, alkylated, and trypsinized before TMT labelling and pooling of the differentially tested lysates to give three conditions (1. NLRP1-FLAG; 2. GFP-FLAG; 3. NLRP1-FLAG+proline) in duplicate. These data represent the results following sample and data processing.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

SUBMITTER: Daniel Ball

LAB HEAD: Daniel A. Bachovchin

PROVIDER: PXD037391 | Pride | 2022-11-04

REPOSITORIES: Pride

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			Action	DRS
	1534AG_S1_DPB_Fr1_i39_DBachovchin.raw	Raw
	1534AG_S1_DPB_Fr2_i39_DBachovchin.raw	Raw
	1534AG_S1_DPB_Fr3_i39_DBachovchin.raw	Raw
	1534AG_S1_DPB_Fr_Peptides.xlsx	Xlsx
	1534AG_S1_DPB_Fr_Proteins.xlsx	Xlsx

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