Proteomics

Dataset Information

0

Proximity labelling with TurboID shows that client proteins of PML NBs differ in three cell contexts


ABSTRACT: PML nuclear body (PML NB) recruits different client proteins under different cell context. We used TurboID proximity labeling (PL) method followed by MS to determine the composition of PML NBs in mESCs, differentiated cells, and NaAsO2-treated mESCs.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Cell Culture, Embryonic Stem Cell

SUBMITTER: Yutong Chen  

LAB HEAD: Yi Lin

PROVIDER: PXD037432 | Pride | 2023-01-10

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
MS_rep1_search_results.xlsx Xlsx
MS_rep2_search_results.xlsx Xlsx
Rep1_Differentiated_DMSO.raw Raw
Rep1_Differentiated_biotin.raw Raw
Rep1_NaAsO2_DMSO.raw Raw
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Publications

Recruitment of TRIM33 to cell-context specific PML nuclear bodies regulates nodal signaling in mESCs.

Sun Hongyao H   Chen Yutong Y   Yan Kun K   Shao Yanqiu Y   Zhang Qiangfeng C QC   Lin Yi Y   Xi Qiaoran Q  

The EMBO journal 20221216 3


TRIM33 is a chromatin reader required for mammalian mesendoderm differentiation after activation of Nodal signaling, while its role in mESCs is still elusive. Here, we report that TRIM33 co-localizes with promyelocytic leukemia nuclear bodies (PML-NBs) specifically in mESCs, to mediate Nodal signaling-directed transcription of Lefty1/2. We show that TRIM33 puncta formation in mESCs depends on PML and on specific assembly of PML-NBs. Moreover, TRIM33 and PML co-regulate Lefty1/2 expression in mES  ...[more]

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