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Proteomics analysis of zebrafish spinal cord regeneration following extracellular matrix manipulations

ABSTRACT: Label-free mass spectrometry-based quantitative proteomics was applied to a larval zebrafish spinal cord injury model, which allows axon regeneration and functional recovery within two days (days post lesion; dpl) after a spinal cord transection in 3 day-old larvae (dpf). Proteomic profiling was performed of the lesion site at 1 dpl in control animals and animals with pdgfrb+ cell-specific overexpression of either zebrafish chondoradherin (chad; chad-mCherry fusion), fibromodulin a (fmoda; fmoda-mCherry fusion), lumican (lum; lum-mCherry fusion) or prolargin (prelp; prelp-mCherry fusion).

INSTRUMENT(S): timsTOF Pro

ORGANISM(S): Danio Rerio (zebrafish) (brachydanio Rerio)

TISSUE(S): Spinal Cord

SUBMITTER: Barbara Steigenberger

LAB HEAD: Dr. Daniel Wehner

PROVIDER: PXD037605 | Pride | 2023-09-06

REPOSITORIES: Pride

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			Action	DRS
	20220322_TTOF01_LC12-19_SA_Orbi4173_Sample3B_E3_1_6574.d.7z	Other
	20220323_TTOF01_LC12-19_SA_Orbi4160_Sample1A_re_F1_1_6409.d.7z	Other
	20220323_TTOF01_LC12-19_SA_Orbi4160_Sample1B_G1_1_6410.d.7z	Other
	20220323_TTOF01_LC12-19_SA_Orbi4160_Sample1C_H1_1_6411.d.7z	Other
	20220323_TTOF01_LC12-19_SA_Orbi4160_Sample2A_F2_1_6413.d.7z	Other

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