Proteomics analysis of plasma-derived exosomes unveils the aberrant complement and coagulation cascades in dermatomyositis/polymyositis

ABSTRACT: Dermatomyositis and polymyositis (DM/PM) are systemic autoimmune diseases characterized by proximal muscle weakness. The underlying pathogenetic mechanism of this disease remains elusive. Here, using proteomics analysis, a great overlap of differentially expressed plasma exosomal proteins involved in the complement and coagulation cascades pathway, including FGA, FGB, FGG, C1QB, C1QC and VWF, were identified in DM/PM patients versus HCs. Correlation analysis revealed that the expression levels of complement-associated proteins (C1QB and C1QC) correlated positively with CRP, ESR and platelet count. ROC curve analysis demonstrated that complement and coagulation cascade-associated proteins could be strong predictors for DM/PM. Additionally, we also identified several other proteins that were differentially expressed in DM and PM, respectively. And the selected candidate proteins were further validated by PRM. Together, these exosome-derived proteins might participate in microvascular damage in DM/PM through the activation of the complement and coagulation cascades pathway and function as biomarkers for the clinical diagnosis of DM/PM.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Blood Plasma

SUBMITTER: Shuhui Meng  

LAB HEAD: Shuhui Meng

PROVIDER: PXD037608 | Pride | 2023-03-11

REPOSITORIES: Pride