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Comparative single-cell transcriptional and proteomic atlas of clinical-grade injectable mesenchymal source tissues

ABSTRACT: Bone marrow aspirate concentrate (BMAC) and adipose-derived stromal vascular fraction (ADSVF) are the most marketed stem cell therapies to treat a variety of conditions in the general population and elite athletes. Both tissues have been used interchangeably clinically even though their detailed composition, heterogeneity, and mechanisms of action have neither been rigorously inventoried nor compared. This lack of information has prevented investigations into ideal dosages and has facilitated anecdata and misinformation. Here, we analyzed single-cell transcriptomes, proteomes, and flow cytometry profiles from paired clinical-grade BMAC and ADSVF. This comparative transcriptional atlas challenges the prevalent notion that there is one therapeutic cell type present in both tissues. We also provide data of surface markers that may enable isolation and investigation of cell (sub)populations. Furthermore, the proteome atlas highlights intertissue and interpatient heterogeneity of injected proteins with potentially regenerative or immunomodulatory capacities.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Bone Marrow, Subcutaneous Adipose Tissue

SUBMITTER: Samuel Ward

LAB HEAD: Samuel Richard

PROVIDER: PXD037632 | Pride | 2024-07-25

REPOSITORIES: Pride

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			Action	DRS
	SR001_fat.csv	Csv
	SR001_fat.mgf	Mgf
	SR001_fat.raw	Raw
	SR002_fat.csv	Csv
	SR002_fat.mgf	Mgf

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