Dataset Information

Effect of knockdEffect of knockdown of mitochondrial atp1 mRNA by a custom-designed PPR protein on protein expression in Arabidopsis thaliana own of mitochondrial atp1 mRNA by a custom-designed PPR protein on protein expression in Arabidopsis thaliana

ABSTRACT: We show that a custom-designed RNA-binding protein binds and specifically induces cleavage of atp1 RNA in mitochondria, significantly decreasing the abundance of the Atp1 protein and the assembled F1Fo ATP synthase in Arabidopsis thaliana. Changes to the proteome as a consequence of the modification were investigated by shotgun proteomics on enriched mitochondrial fractions.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Arabidopsis Thaliana (mouse-ear Cress)

TISSUE(S): Plant Cell, Photosynthetic Cell, Leaf

SUBMITTER: Elke Stroeher

LAB HEAD: Ian Small

PROVIDER: PXD037659 | Pride | 2023-10-24

REPOSITORIES: Pride

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Alteration of Mitochondrial Transcript Expression in <i>Arabidopsis thaliana</i> Using a Custom-Made Library of Pentatricopeptide Repeat Proteins.

Vincis Pereira Sanglard Lilian L Small Ian D ID Colas des Francs-Small Catherine C

International journal of molecular sciences 20230826 17

Pentatricopeptide repeat (PPR) proteins are considered a potential tool for manipulating organelle gene expression in plants because they can recognise a wide range of different RNA sequences, and the molecular basis for this sequence recognition is partially known and understood. A library of redesigned PPR proteins related to restorer-of-fertility proteins was created and transformed into plants in order to target mitochondrial transcripts. Ninety different variants tested in vivo showed a wid ...[more]

PMID: 37686040

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