Proteomics

Dataset Information

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The TriMet_DB: a manually curated database of the metabolic proteins of Triticum aestivum


ABSTRACT: Mass spectrometry-based wheat proteomics is challenging because the current interpretation of mass spectrometry data relies on public databases that are not exhaustive (UniProtKB/Swiss-Prot) or contain many redundant and poor or un-annotated entries (UniProtKB/TrEMBL). Here we report the development of a manually curated database of the metabolic proteins of Triticum aestivum (hexaploid wheat), named TriMet_DB (Triticum aestivum Metabolic Proteins DataBase). The manually curated TriMet_DB was generated in FASTA format, so that it can be read directly by programs used to interpret the mass spectrometry data. Furthermore, the complete list of entries included in the TriMet_DB is reported in a freely available resource, which includes for each protein the description, the gene code, the protein family,and the allergen name (if any). To evaluate its performance, the TriMet_DB was used to interpret the mass spectrometry data acquired on the metabolic protein fraction extracted from the MEC cultivar of Triticum aestivum.

INSTRUMENT(S): Orbitrap Fusion

ORGANISM(S): Triticum Aestivum (wheat)

SUBMITTER: Antonella Di Francesco  

LAB HEAD: Vincenzo Cunsolo

PROVIDER: PXD037709 | Pride | 2023-03-11

REPOSITORIES: Pride

Dataset's files

Source:
Action DRS
Met_00003567.raw Raw
Met_00003568.raw Raw
Met_00003569.raw Raw
protein-peptides_SW_DB.csv Csv
protein-peptides_TriMet_DB.csv Csv
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Publications

The TriMet_DB: A Manually Curated Database of the Metabolic Proteins of <i>Triticum aestivum</i>.

Cunsolo Vincenzo V   Di Francesco Antonella A   Pittalà Maria Gaetana Giovanna MGG   Saletti Rosaria R   Foti Salvatore S  

Nutrients 20221218 24


Mass-spectrometry-based wheat proteomics is challenging because the current interpretation of mass spectrometry data relies on public databases that are not exhaustive (UniProtKB/Swiss-Prot) or contain many redundant and poor or un-annotated entries (UniProtKB/TrEMBL). Here, we report the development of a manually curated database of the metabolic proteins of <i>Triticum aestivum</i> (hexaploid wheat), named TriMet_DB (Triticum aestivum Metabolic Proteins DataBase). The manually curated TriMet_D  ...[more]

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