Proteomics

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Integrating intracellular and extracellular proteomic profiling for in-depth investigations of cellular communication in a model of prostate cancer


ABSTRACT: Cellular communication is essential for cell-cell interactions, maintaining homeostasis and progression of certain disease states. While many studies examine extracellular proteins, the holistic extracellular proteome is often left uncaptured, leaving gaps in our understanding of how all extracellular proteins may impact communication and interaction. We used a cellular-based proteomics approach to more holistically profile both the intracellular and extracellular proteome of prostate cancer. Our workflow was generated in such a manner that multiple experimental conditions can be observed with the opportunity for high throughput integration. Additionally, this workflow is not limited to a proteomic aspect, as metabolomic and lipidomic studies can be integrated for a multi-omics workflow. Our analysis showed coverage of over 8000 proteins while also garnering insights into cellular communication in the context of prostate cancer development and progression. Identified proteins covered a variety of cellular processes and pathways, allowing for investigation of multiple aspects into cellular biology. This workflow demonstrates advantages for integrating intra- and extracellular proteomic analyses as well as potential for multi-omics researchers. This approach possesses great value for future investigations into the systems biology aspects of disease development and progression.

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Epithelial Cell, Cell Culture

DISEASE(S): Prostate Adenocarcinoma

SUBMITTER: Hannah Miles  

LAB HEAD: Lingjun Li

PROVIDER: PXD037806 | Pride | 2023-05-25

REPOSITORIES: Pride

Dataset's files

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20220523_HM_MT10_DMSO-1_R1.raw Raw
20220523_HM_MT10_DMSO-1_R2.raw Raw
20220523_HM_MT10_DMSO-1_R3.raw Raw
20220523_HM_MT10_DMSO-2_R1.raw Raw
20220523_HM_MT10_DMSO-2_R2.raw Raw
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Publications

Integrating intracellular and extracellular proteomic profiling for in-depth investigations of cellular communication in a model of prostate cancer.

Miles Hannah N HN   Tomlin Devin D   Ricke William A WA   Li Lingjun L  

Proteomics 20230524 21-22


Cellular communication is essential for cell-cell interactions, maintaining homeostasis and progression of certain disease states. While many studies examine extracellular proteins, the holistic extracellular proteome is often left uncaptured, leaving gaps in our understanding of how all extracellular proteins may impact communication and interaction. We used a cellular-based proteomics approach to more holistically profile both the intracellular and extracellular proteome of prostate cancer. Ou  ...[more]

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