Proteomics

Dataset Information

0

Proteomics of Mouse Tumor ECM LC-MS/MS

ABSTRACT: Type I, II, III and V collagens were commonly identified in human, pig, and mouse breast ECM. Mammary epithelial cells were able to form acini on certain types or combinations of the four collagens at normal breast tissue stiffness levels. Comparison of the collagen species in mouse normal breast and breast tumor ECM revealed common and distinct sets of collagens within the two types of tissues. Elevated collagen type I alpha 1 chain expression was found in human breast cancers. Collagen type XXV alpha 1 chain was identified in mouse breast tumors but not in normal breast tissues. Our data provide insights into modeling human breast pathophysiological structures and functions using native tissue-derived hydrogels and potential contributions of different collagen types or their monomers in breast cancer development.

INSTRUMENT(S): Agilent instrument model

ORGANISM(S): Homo Sapiens (human)

TISSUE(S): Breast

SUBMITTER: Weimin Li  

LAB HEAD: Weimin Li

PROVIDER: PXD037920 | Pride | 2023-03-11

REPOSITORIES: Pride

Json Xml

Dataset's files

Source:
Action DRS
ECM-T_WSU.raw Raw
ECM-T_WSU__F017701_.mzid.gz Mzid
ECM-T_WSU__F017701_.mzid_ECM-T_WSU__F017701_.MGF Mzid
Items per page:
1 - 3 of 3
altmetric image

Publications

Identification of the Collagen Types Essential for Mammalian Breast Acinar Structures.

Keller Chandler R CR   Ruud Kelsey F KF   Martinez Steve R SR   Li Weimin W  

Gels (Basel, Switzerland) 20221218 12

Modeling human breast tissue architecture is essential to study the pathophysiological conditions of the breast. We report that normal mammary epithelial cells grown in human breast extracellular matrix (ECM) hydrogel formed acini structurally similar to those of human and pig mammary tissues. Type I, II, III and V collagens were commonly identified in human, pig, and mouse breast ECM. Mammary epithelial cells formed acini on certain types or combinations of the four collagens at normal levels o  ...[more]

Similar Datasets

Proteomics Proteomics of Human Breast-TMG LC-MS/MS
2023-03-11 | PXD037900 | Pride
Proteomics Characterization of the Chemotherapy-treated PyMT Matrisome
2022-03-14 | PXD032002 | Pride
Proteomics Murine Liver tissue on high fat diet
2024-06-06 | PXD051409 | Pride
Proteomics Characterization of Chemotherapy-treated PyMT Liver Matrisome
2022-10-05 | PXD036736 | Pride
Proteomics Murine Mammary fat pad on high fat diet
2024-06-06 | PXD050250 | Pride
Proteomics Dental Pulp Stem Cells LC-MS/MS analysis
2021-09-23 | PXD018951 | Pride
Proteomics Proteomic analysis of extracellular matrix remodeling during zebrafish heart regeneration
2019-08-26 | PXD010092 | Pride
Proteomics A Pluripotent Stem Cell-Derived Matrix with Potent Osteoregenerative Capabilities
2020-07-02 | PXD016017 | Pride
Transcriptomics The comparison of exosomal miRNAs from ECM or media derived from HFF-CBD-Lamp2b cells, HFF cells, HFF-Lamp2b cells and hUCMSCs
2021-10-12 | E-MTAB-10905 | biostudies-arrayexpress
Proteomics In depth quantification of extracellular matrix proteins from human pancreas
2019-06-17 | PXD012460 | Pride