Dataset Information

Dataset from The proteome landscape of the black soldier fly provides insights into protein function and microbial diversity

ABSTRACT: The larvae of black soldier fly (BSF) Hermetia illucens (Diptera: Stratiomyidae), has demonstrated ability in the efficient bioconversion of organic waste into a sustainable source of food and feed, but fundamental biology remains to be discovered to exploit their full biodegradative potential. Herein, LC-MS/MS was used to assess the efficiency of eight differing extraction protocols to build foundational knowledge regarding the proteome landscape of both BSF larvae body and gut. No specific protocol was superior in capturing the BSF body and gut proteome, but each yielded complementary information to improve BSF proteome coverage. Protocol-specific functional annotation using protein level information has shown that the selection of extraction buffer can affect protein detection and their associated functional classes within the measured BSF larval gut proteome. Metaproteome analysis on BSF larvae gut has uncovered the prevalence of two bacterial phyla: actinobacteria and proteobacteria. We envisage that comparing a range of extraction protocols and investigating the proteome from the BSF body and gut separately will expand the fundamental knowledge of the BSF proteome and thereby provide translational opportunities for future research to enhance their efficiency for waste degradation and contribution to the circular economy.

INSTRUMENT(S): TripleTOF 5600

ORGANISM(S): Hermetia Illucens

TISSUE(S): Gut

SUBMITTER: Sophia Escobar Correas

LAB HEAD: Utpal Bose

PROVIDER: PXD037977 | Pride | 2023-03-11

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	BSF_Gut_Combined-search.group	Other
	BSF_Gut_Combined-search__FDR.xlsx	Xlsx
	Protein_Alignment_Template_Bead_UTC_Defat-Fat.xlsx	Xlsx
	Protein_Alignment_Template_Bead_tris_Defat-fat.xlsx	Xlsx
	Protein_Alignment_Template_N2_UTC_Defat-fat.xlsx	Xlsx

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Publications

Unpacking the Proteome and Metaproteome of the Black Soldier Fly Larvae: Efficacy and Complementarity of Multiple Protein Extraction Protocols.

Bose Utpal U Juhasz Angela A Stockwell Sally S Escobar-Correas Sophia S Marcora Anna A Paull Cate C Broadbent James A JA Wijffels Gene G

ACS omega 20230220 8

The larvae of the black soldier fly (BSF), <i>Hermetia illucens</i> (Diptera: Stratiomyidae), have demonstrated the ability to efficiently bioconvert organic waste into a sustainable source of food and feed, but fundamental biology remains to be discovered to exploit their full biodegradative potential. Herein, LC-MS/MS was used to assess the efficiency of eight differing extraction protocols to build foundational knowledge regarding the proteome landscape of both the BSF larvae body and gut. Ea ...[more]

PMID: 36872973

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