Dataset Information

Opisthorchis felineus excretory-secretory product (ESP) and lysate proteomics by GeLC-MS / MS

ABSTRACT: A significant number of pathological conditions, accompanied by chronic non-healing wounds, demands searching for new modern therapeutic approaches. Well-documented ability of O. felineus to initiate extracellular matrix (ECM) remodeling and liver epithelium regeneration suggests that its bioactive molecules may stimulate skin wound healing processes. The aim of this study was to investigate the wound healing potential of the Opisthorchis felineus excretory-secretory and lysate proteins on a murine model. The following methods were used for the study: histological (wounded skin condition), immunohistochemical (ECM, neoangiogenesis, O. felineus GST and TPx proteins), gene expression analysis (inflammation, angiogenesis, ECM condition). O. felineus excretory-secretory product (ESP) and lysate proteins have revealed wound-healing potential, they: i) reduce inflammation levels, ii) modulate vascular response, iii) stimulate collagen deposition and dermal ECM remodeling. Additional proteomic analysis of adult O. felineus ESP and lysate samples was conducted. Proteomic analysis approach called GeLC-MS / MS was chosen to study of the excretory-secretory product and lysate proteins. This approach is based on one-dimensional sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), in-gel protein digestion with trypsin followed by liquid chromatography-tandem mass spectrometry. The SDS-PAGE step allow to removes hemozoin also as detergents, buffers and salts from the protein extract that may interfere with mass spectrometry analysis

INSTRUMENT(S): Q Exactive HF

ORGANISM(S): Mus Musculus (mouse)

SUBMITTER: Olga Tikhonova

LAB HEAD: Olga Tikhonoiva

PROVIDER: PXD037991 | Pride | 2023-03-11

REPOSITORIES: Pride

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			Action	DRS
	180222CKP_Paxarukova01_20220221082625.raw	Raw
	180222CKP_Paxarukova01_20220221095946.raw	Raw
	180222CKP_Paxarukova02.raw	Raw
	180222CKP_Paxarukova02_20220221140821.raw	Raw
	180222CKP_Paxarukova02_20220221154140.raw	Raw

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Publications

Wound healing approach based on excretory-secretory product and lysate of liver flukes.

Kovner Anna V AV Tarasenko Alena A AA Zaparina Oxana O Tikhonova Olga V OV Pakharukova Maria Y MY Mordvinov Viatcheslav A VA

Scientific reports 20221214 1

Exogenous bioactive peptides are considered promising for the wound healing therapy in humans. In this regard, parasitic trematodes proteins may potentially become a new perspective agents. Foodborne trematode Opisthorchis felineus is widespread in Europe and has the ability to stimulate proliferation of bile duct epithelium. In this study, we investigated skin wound healing potential of O. felineus proteins in mouse model. C57Bl/6 mice were inflicted with superficial wounds with 8 mm diameter. ...[more]

PMID: 36517588

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