Proteomics

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Identifying the glycoproteome of Burkholderia cenocepacia following knockdown of glycosylation using CRISPR interference (low dCas9 induction)

ABSTRACT: To interrogate the glycoproteome following knockdown of pglL and subsequent knockdown of glycosylation in Burkholderia cenocepacia through Zwitterionic Hydrophilic Interaction Liquid Chromatography (ZIC-HILIC) glycopeptide enrichment with 0.1% rhamnose for dCas9 induction. Using strains harbouring the pglL-targeting guide C (Bc-pglL-C) and guideless plasmids.

INSTRUMENT(S): Orbitrap Fusion Lumos

ORGANISM(S): Burkholderia Cenocepacia K56-2valvano

SUBMITTER: Jessica Lewis  

LAB HEAD: Nichollas Scott

PROVIDER: PXD038047 | Pride | 2023-03-20

REPOSITORIES: Pride

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Dataset's files

Source:
Action DRS
20220921_JL-4910_BC_glycopeptide_CRISPRi_I_Guide_C_A.raw Raw
20220921_JL-4910_BC_glycopeptide_CRISPRi_I_Guide_C_B.raw Raw
20220921_JL-4910_BC_glycopeptide_CRISPRi_I_Guide_C_C.raw Raw
20220921_JL-4910_BC_glycopeptide_CRISPRi_I_Guide_C_D.raw Raw
20220921_JL-4910_BC_glycopeptide_CRISPRi_I_Guideless_A.raw Raw
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