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Combined effects of EMP and RF exposure on mouse hippocampus LC-MSMS

ABSTRACT: Recently, concerns about the combined effects of electromagnetic field (EMF) in daily living and occupational environment are rapidly growing. 4.9 GHz radiofrequency (RF) is commonly used in 5G telecommunication in daily life, EMP is common is occupational environment. Till now, the biological effects of combined exposure to EMP and 4.9 GHz RF have not been fully understood. We used LC-MSMS quantitative proteomics method to investigate the potential effects of EMP and RF field exposure in adult male mice model.

INSTRUMENT(S): Orbitrap Exploris 480

ORGANISM(S): Mus Musculus (mouse)

TISSUE(S): Hippocampus

SUBMITTER: Guirong Ding

LAB HEAD: Tongzhou Qin

PROVIDER: PXD038101 | Pride | 2023-05-10

REPOSITORIES: Pride

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Publications

Combined effects of EMP and RF field on emotional behavior in mice.

Qin Tongzhou T Liu Liyuan L Wang Xing X Guo Ling L Lin Jiajin J Du Junze J Xue Yizhe Y Lai Panpan P Jing Yuntao Y Ding Guirong G

Frontiers in public health 20230316

<h4>Background</h4>Recently, concerns about the combined effects of electromagnetic field (EMF) in daily living and occupational environment are rapidly growing.<h4>Methods</h4>In this study, we investigated the combined effects of 1-week exposure to electromagnetic pulse (EMP) at 650 kV/m for 1,000 pulses and 4.9 GHz radiofrequency (RF) at 50 W/m<sup>2</sup> for 1 h/d in male mice. Open field test, tail suspension test and Y-maze were applied to evaluate anxiety, depression-like behaviors and s ...[more]

PMID: 37006533

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Project description:<p>With the rapid development of 5G networks, the influence of the radiofrequency field (RF) generated from 5G communication equipment on human health are gathering increasing attention. This study aimed at assessing the effects of long-term exposure to 4.9 GHz (one of the working frequency of 5G communication) RF field on fecal microbiome and metabolome profiles in 9-week-old male C57BL/6 mice. The animals were divided into sham group (n = 8) and radiofrequency group (RF group; n = 8), then whole body exposed or sham exposed to RF field for one hour per day during 3 weeks (21 days) at whole body Specific Absorption Rate (SAR) of 6.3 W/kg. After RF exposure, the mice fecal samples were collected to detect gut microorganisms and metabolites by 16S rRNA gene sequencing method and LC-MS method, respectively. The results showed that intestinal microbial compositions were altered in RF group, as evidenced by reduced microbial diversity and changed microbial community distribution. Metabolomics profiling identified 258 significantly differentially abundant metabolites in RF group, 57 of which can be classified to Kyoto Encyclopedia of Genes and Genomes (KEGG) pathways. Besides, functional correlation analysis showed that changes in gut microbiota genera were significantly correlated with changes in fecal metabolites. In summary, the results suggested that altered gut microbiota and metabolic profile are associated with 4.9 GHz radiofrequency exposure.</p>

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Project description:This study assessed changes in gene expression in 7 mouse brain regions using Illumina Mouse WG-6 (v2) beadchips after exposure to 1.9 GHz pulse-modulated radiofrequency field exposure for 4h/day for 5 days. High dose RF field exposure was whole body average SAR and brain average SAR were 1.45 W/kg and 0.16 W/kg, respectively. Total RNA isolated from the amygdala, auditory cortex, caudate, cerebellum, hippocampus, hypothalamus and medial prefrontal cortex of 1.9 GHz pulse-modulated radiofrequency field exposed mice and compared to that in sham (handled) exposed animals. An unhandled control group was also included in the study design. A total of 20 RNA samples (5 independent biological tissues for each of 4 treatment groups) were collected for each brain region. When analysis of gene expression was conducted within individual brain regions when controlling the false discovery rate (FDR), no differentially expressed genes were identified relative to the sham control. However, it must be noted that most fold changes among groups were observed to be less than 1.5 fold and this study had limited ability to detect such small changes. While some genes were differentially expressed without correction for multiple-comparisons testing, no consistent pattern of response was observed among different RF-exposure levels or among different RF-modulations. The current study provides the most comprehensive analysis of potential gene expression changes in the rodent brain in response to RF field exposure conducted to date. Within the exposure conditions and limitations of this study, no convincing evidence of consistent changes in gene expression was found in response to 1.9 GHz pulse-modulated RF field exposure.

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