Dataset Information

A differential proteomic approach highlighted differences induced by plant growth promoting bacteria in Solanum lycopersicum treated with Kocuria rhizophila and Streptomyces violaceoruber

ABSTRACT: During the last decades, the use of plant growth promoting bacteria (PGPB) has been found to increase crop yield and quality and to confer abiotic and biotic stress tolerance. However, until now the PGPB mechanism to enhance plant performances is not clearly defined. Recently, our findings demonstrated that inoculations with both Kocuria rhizophila and Streptomyces violaceoruber, as well as their combination, determined an increase of tomato (Solanum lycopersicum) growth and development. In this study, through an advanced differential proteomic approach on tomato leaves, plant molecular mechanisms affected by both K. rhizophila and S. violaceoruber have been elucidated. To this aim, tomato plants were treated with K. rhizophila and/or Streptomyces violaceoruber cultures and grown on coconut fiber in greenhouse. In particular, PGPB treatments were conducted twice, on seed and after two weeks from the seedling by fertirrigation. Thus, the analyses have been performed at 14 days after sowing (DAS) (T1) and 42 DAS (T2). The results confirmed the growth stimulation ability of K. rhizophila/Streptomyces violaceoruber, showing shoot fresh and dry weight significantly improved at each time sampling. For the early phase (DAS-T1) comparative proteomics analysis of tomato plant leaves, 2 biological replicates were set up for the plants used as control (i.e. not subjected to treatment - samples I1 and I2-control I), 2 biological replicates for plants subjected to treatment with K. rhizophila (samples L1 and L2-treatment L), 2 biological replicates for plants subjected to treatment with S. violaceoruber (samples M1 and M2-treatment M), and 2 biological replicates for plants subjected to treatment with a mix of the two bacterial strains (samples N1 and N2-treatment N), for a total of 8 samples of leaf protein extracts. For the late phase (DAS-T2) comparative proteomics analysis of tomato plant leaves, 2 biological replicates were set up for the plants used as control (i.e. not treated - samples A1 and A2 - control A), 2 biological replicates for plants subjected to treatment with K. rhizophila (samples B1 and B2-treatment B), 2 biological replicates for plants subjected to treatment with S. violaceoruber (samples C1 and C2-treatment C), and 2 biological replicates for plants subjected to treatment with a mix of the two bacterial strains (samples D1 and D2-treatment D), for a total of 8 samples of leaf protein extracts. Proteomic analysis was able to identify 239 and 203 significantly differentially represented proteins (DRPs) at T1 and T2, respectively, comparing PGPB-treated vs. untreated control plants. KEGG Orthology (KO) identified DRP belonging to photosynthesis, biosynthesis of secondary metabolites, and carbon metabolism.

INSTRUMENT(S): Q Exactive Plus

ORGANISM(S): Solanum Lycopersicum

TISSUE(S): Plant Cell, Leaf

SUBMITTER: Giovanni Renzone

LAB HEAD: Andrea Scaloni

PROVIDER: PXD038137 | Pride | 2024-05-02

REPOSITORIES: Pride

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Dataset's files

Source:

			Action	DRS
	Leaves_EarlyStages_a-_1_.msf	Msf
	Leaves_EarlyStages_a1.raw	Raw
	Leaves_EarlyStages_a2.raw	Raw
	Leaves_EarlyStages_a3.raw	Raw
	Leaves_EarlyStages_a4.raw	Raw

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Publications

Integrated omics approach reveals the molecular pathways activated in tomato by Kocuria rhizophila, a soil plant growth-promoting bacterium.

Mauceri Antonio A Puccio Guglielmo G Faddetta Teresa T Abbate Loredana L Polito Giulia G Caldiero Ciro C Renzone Giovanni G Lo Pinto Margot M Alibrandi Pasquale P Vaccaro Edoardo E Abenavoli Maria Rosa MR Scaloni Andrea A Sunseri Francesco F Cavalieri Vincenzo V Palumbo Piccionello Antonio A Gallo Giuseppe G Mercati Francesco F

Plant physiology and biochemistry : PPB 20240413

Plant microbial biostimulants application has become a promising and eco-friendly agricultural strategy to improve crop yields, reducing chemical inputs for more sustainable cropping systems. The soil dwelling bacterium Kocuria rhizophila was previously characterized as Plant Growth Promoting Bacteria (PGPB) for its multiple PGP traits, such as indole-3-acetic acid production, phosphate solubilization capability and salt and drought stress tolerance. Here, we evaluated by a multi-omics approach, ...[more]

PMID: 38615442

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