Proteomics

Dataset Information

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Protein composition of extracellular vesicles isolated from Haloferax volcanii

ABSTRACT: In order to understand extraceluular vesicle (EV) production in Euryarchaea, and in particular halophilic archaea, we used the model archaeal organism, Haloferax volcanii, to investigate the composition of EVs as well as their capacity to transfer their cargo to other organisms. EVs were isolated in tripclicates and purified with a density gradient. Both of two bands (upper and lower band) were isolated from the gradient and analysed seperately. Additionally, cellular membranes were isolated in triplicates and analysed for their protein content, to allow conclusions about proteins that are preferentially enclosed in EVs.

INSTRUMENT(S): LTQ Orbitrap Velos, Q Exactive

ORGANISM(S): Haloferax Volcanii Ds2

SUBMITTER: Susanne Erdmann  

LAB HEAD: Susanne Erdmann

PROVIDER: PXD038319 | Pride | 2024-05-24

REPOSITORIES: Pride

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20200603_A1.mzid.gz Mzid
20200603_A1.mzid_20200603_A1.MGF Mzid
20200603_A2.mzid.gz Mzid
20200603_A2.mzid_20200603_A2.MGF Mzid
20200603_A3.mzid.gz Mzid
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Publications

Extracellular vesicle formation in <i>Euryarchaeota</i> is driven by a small GTPase.

Mills Joshua J   Gebhard L Johanna LJ   Schubotz Florence F   Shevchenko Anna A   Speth Daan R DR   Liao Yan Y   Duggin Iain G IG   Marchfelder Anita A   Erdmann Susanne S  

Proceedings of the National Academy of Sciences of the United States of America 20240226 10

Since their discovery, extracellular vesicles (EVs) have changed our view on how organisms interact with their extracellular world. EVs are able to traffic a diverse array of molecules across different species and even domains, facilitating numerous functions. In this study, we investigate EV production in <i>Euryarchaeota,</i> using the model organism <i>Haloferax volcanii</i>. We uncover that EVs enclose RNA, with specific transcripts preferentially enriched, including those with regulatory po  ...[more]

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