Dataset Information

The central mediator of auxin-triggered phosphorylation in plants is a RAF-like kinase.

ABSTRACT: The plant signaling molecule auxin triggers both fast and slow cellular responses across the plant lineage, including both land plants and algae. We discovered an ultra-fast proteome-wide phosphorylation response to auxin across 5 land plant and algal species, converging on a core group of shared target proteins. We find conserved rapid physiological responses to auxin in the same species and identified a RAF-like protein kinase as a central mediator of auxin-triggered phosphorylation across species.

INSTRUMENT(S): Q Exactive HF-X, Orbitrap Exploris 480

ORGANISM(S): Penium Margaritaceum Physcomitrella Patens Subsp. Patens (moss) Marchantia Polymorpha Arabidopsis Thaliana (mouse-ear Cress) Klebsormidium Nitens

TISSUE(S): Root, Thallus, Cell Culture

SUBMITTER: Sjef Boeren

LAB HEAD: Dolf Weijers

PROVIDER: PXD038363 | Pride | 2024-01-26

REPOSITORIES: pride

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Dataset's files

Source:

			Action	DRS
	MQresult_MR1969-1992.zip	Other
	MQresult_MR2119-2126_.zip	Other
	MQresult_MR2127-2134.zip	Other
	MQresult_MR2157a-2164.zip	Other
	MQresult_MR2509-2512_2521-2524_2533-2540.zip	Other

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Publications

RAF-like protein kinases mediate a deeply conserved, rapid auxin response.

Kuhn Andre A Roosjen Mark M Mutte Sumanth S Dubey Shiv Mani SM Carrillo Carrasco Vanessa Polet VP Boeren Sjef S Monzer Aline A Koehorst Jasper J Kohchi Takayuki T Nishihama Ryuichi R Fendrych Matyáš M Sprakel Joris J Friml Jiří J Weijers Dolf D

Cell 20231220 1

The plant-signaling molecule auxin triggers fast and slow cellular responses across land plants and algae. The nuclear auxin pathway mediates gene expression and controls growth and development in land plants, but this pathway is absent from algal sister groups. Several components of rapid responses have been identified in Arabidopsis, but it is unknown if these are part of a conserved mechanism. We recently identified a fast, proteome-wide phosphorylation response to auxin. Here, we show that t ...[more]

PMID: 38128538

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